<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2024 Workiva-->
<!--r:6e36f738-c3ff-46f5-a556-0a3546ed873d,g:503e3b70-be60-4d1e-9e8e-7891fa4d9594-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://cytekbio.com/role/ConsolidatedBalanceSheets" xlink:type="simple" xlink:href="ctkb-20240930.xsd#ConsolidatedBalanceSheets"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/ConsolidatedBalanceSheets" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_f20a931f-885a-4396-ae36-04a08bdbbe75" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableTradeCurrent_0522482d-7899-489e-9d7d-4db427c1fa2e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsPayableTradeCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_f20a931f-885a-4396-ae36-04a08bdbbe75" xlink:to="loc_us-gaap_AccountsPayableTradeCurrent_0522482d-7899-489e-9d7d-4db427c1fa2e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SettlementLiabilitiesCurrent_5225db73-5f3c-4427-bdfb-6a48bd2ecdeb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SettlementLiabilitiesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_f20a931f-885a-4396-ae36-04a08bdbbe75" xlink:to="loc_us-gaap_SettlementLiabilitiesCurrent_5225db73-5f3c-4427-bdfb-6a48bd2ecdeb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_d57bd4e5-1ccf-4b6a-9ec9-1ea421dd7b3d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_f20a931f-885a-4396-ae36-04a08bdbbe75" xlink:to="loc_us-gaap_AccruedLiabilitiesCurrent_d57bd4e5-1ccf-4b6a-9ec9-1ea421dd7b3d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesCurrent_aac4a711-a83f-4db1-8a09-3455f08eb4ed" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLiabilitiesCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_f20a931f-885a-4396-ae36-04a08bdbbe75" xlink:to="loc_us-gaap_OtherLiabilitiesCurrent_aac4a711-a83f-4db1-8a09-3455f08eb4ed" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredRevenueCurrent_cd56d45c-e5c5-4dc1-860c-1c155f490732" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredRevenueCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_f20a931f-885a-4396-ae36-04a08bdbbe75" xlink:to="loc_us-gaap_DeferredRevenueCurrent_cd56d45c-e5c5-4dc1-860c-1c155f490732" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_41d0ef14-77b2-4e40-a9d0-ea967011187c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_7a602e07-4d46-47af-9bec-3731295dfaa9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Liabilities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_41d0ef14-77b2-4e40-a9d0-ea967011187c" xlink:to="loc_us-gaap_Liabilities_7a602e07-4d46-47af-9bec-3731295dfaa9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingencies_e480f0f5-228c-4a93-8b56-ff8c82ec1e2b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommitmentsAndContingencies"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_41d0ef14-77b2-4e40-a9d0-ea967011187c" xlink:to="loc_us-gaap_CommitmentsAndContingencies_e480f0f5-228c-4a93-8b56-ff8c82ec1e2b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_26584747-91a2-4947-9bc1-fb900a68022c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_41d0ef14-77b2-4e40-a9d0-ea967011187c" xlink:to="loc_us-gaap_StockholdersEquity_26584747-91a2-4947-9bc1-fb900a68022c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_cc2d8d97-1c82-4012-84c1-f22c3601a44f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Assets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_da3be3ba-b9ed-408c-835a-5982408a61ce" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_cc2d8d97-1c82-4012-84c1-f22c3601a44f" xlink:to="loc_us-gaap_AssetsCurrent_da3be3ba-b9ed-408c-835a-5982408a61ce" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsDeferredIncome_64645731-a648-400b-bb7c-6c6ef347bd54" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsDeferredIncome"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_cc2d8d97-1c82-4012-84c1-f22c3601a44f" xlink:to="loc_us-gaap_DeferredTaxAssetsDeferredIncome_64645731-a648-400b-bb7c-6c6ef347bd54" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_1a90bc87-8fe3-40f4-b25f-e75789c9887a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_cc2d8d97-1c82-4012-84c1-f22c3601a44f" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_1a90bc87-8fe3-40f4-b25f-e75789c9887a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_85b3d3fa-a7bc-45b2-934a-95811bfb5734" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_cc2d8d97-1c82-4012-84c1-f22c3601a44f" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_85b3d3fa-a7bc-45b2-934a-95811bfb5734" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_363778ad-ef7f-47d2-ab1b-10dd903c8401" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Goodwill"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_cc2d8d97-1c82-4012-84c1-f22c3601a44f" xlink:to="loc_us-gaap_Goodwill_363778ad-ef7f-47d2-ab1b-10dd903c8401" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_238eb876-0177-403f-9135-7fc4d2d492d9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IntangibleAssetsNetExcludingGoodwill"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_cc2d8d97-1c82-4012-84c1-f22c3601a44f" xlink:to="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_238eb876-0177-403f-9135-7fc4d2d492d9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_05e3502d-3a7c-4cac-8277-822d410decd9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_cc2d8d97-1c82-4012-84c1-f22c3601a44f" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_05e3502d-3a7c-4cac-8277-822d410decd9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_8633eba1-b05b-4065-bf41-0383bd047b94" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Liabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_b6637814-47a7-4646-8a08-67c5f4f63b13" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_8633eba1-b05b-4065-bf41-0383bd047b94" xlink:to="loc_us-gaap_LiabilitiesCurrent_b6637814-47a7-4646-8a08-67c5f4f63b13" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LitigationReserveNoncurrent_9a3cf83a-2a8a-4b3a-b430-c787b2fbf5fe" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LitigationReserveNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_8633eba1-b05b-4065-bf41-0383bd047b94" xlink:to="loc_us-gaap_LitigationReserveNoncurrent_9a3cf83a-2a8a-4b3a-b430-c787b2fbf5fe" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredRevenueNoncurrent_0d6592f7-4d9b-4ac0-896c-4a20475f04b8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredRevenueNoncurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_8633eba1-b05b-4065-bf41-0383bd047b94" xlink:to="loc_us-gaap_DeferredRevenueNoncurrent_0d6592f7-4d9b-4ac0-896c-4a20475f04b8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_aeb629e2-be66-4a31-ac3c-1431cebb064b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_8633eba1-b05b-4065-bf41-0383bd047b94" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_aeb629e2-be66-4a31-ac3c-1431cebb064b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_ecb0dfa6-b05e-4efd-8f2a-f0dee941fd23" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_8633eba1-b05b-4065-bf41-0383bd047b94" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_ecb0dfa6-b05e-4efd-8f2a-f0dee941fd23" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_4c03677a-91b0-40a7-8fec-828741be207e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_8633eba1-b05b-4065-bf41-0383bd047b94" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_4c03677a-91b0-40a7-8fec-828741be207e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_af35b4a9-df7b-48ff-9d77-ce23e8bb5111" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_6a175aa2-7285-4cd7-9a40-b8c5ab139932" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_af35b4a9-df7b-48ff-9d77-ce23e8bb5111" xlink:to="loc_us-gaap_CommonStockValue_6a175aa2-7285-4cd7-9a40-b8c5ab139932" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_71c12d2f-d4cf-4a93-a867-449467c3d270" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_af35b4a9-df7b-48ff-9d77-ce23e8bb5111" xlink:to="loc_us-gaap_AdditionalPaidInCapital_71c12d2f-d4cf-4a93-a867-449467c3d270" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_df004f78-f1a4-4979-9aed-2c3679cd73d5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_af35b4a9-df7b-48ff-9d77-ce23e8bb5111" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_df004f78-f1a4-4979-9aed-2c3679cd73d5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_0ecd304e-bed1-498f-ba67-af85ca5945ae" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_af35b4a9-df7b-48ff-9d77-ce23e8bb5111" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_0ecd304e-bed1-498f-ba67-af85ca5945ae" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_96ae928f-0d18-483c-85c5-71e489c0d791" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_3f755470-376c-4b24-8fec-7b22922b3c95" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_96ae928f-0d18-483c-85c5-71e489c0d791" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_3f755470-376c-4b24-8fec-7b22922b3c95" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableNetCurrent_0e7658ef-391f-4248-971e-e37e64ddfdda" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsReceivableNetCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_96ae928f-0d18-483c-85c5-71e489c0d791" xlink:to="loc_us-gaap_AccountsReceivableNetCurrent_0e7658ef-391f-4248-971e-e37e64ddfdda" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashCurrent_4e507bf6-68c2-4b00-b18d-adf67b0125ba" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_96ae928f-0d18-483c-85c5-71e489c0d791" xlink:to="loc_us-gaap_RestrictedCashCurrent_4e507bf6-68c2-4b00-b18d-adf67b0125ba" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MarketableSecurities_f581f737-9f35-4525-a5f9-9e26cabbf3df" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_MarketableSecurities"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_96ae928f-0d18-483c-85c5-71e489c0d791" xlink:to="loc_us-gaap_MarketableSecurities_f581f737-9f35-4525-a5f9-9e26cabbf3df" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_bb7badb5-d27e-4aa8-a2ae-bce9f93496d6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InventoryNet"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_96ae928f-0d18-483c-85c5-71e489c0d791" xlink:to="loc_us-gaap_InventoryNet_bb7badb5-d27e-4aa8-a2ae-bce9f93496d6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_802f9eda-65dd-467a-ba6f-79464dd3dcb8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_96ae928f-0d18-483c-85c5-71e489c0d791" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_802f9eda-65dd-467a-ba6f-79464dd3dcb8" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/ConsolidatedStatementsofOperationsandComprehensiveIncomeLossunaudited" xlink:type="simple" xlink:href="ctkb-20240930.xsd#ConsolidatedStatementsofOperationsandComprehensiveIncomeLossunaudited"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/ConsolidatedStatementsofOperationsandComprehensiveIncomeLossunaudited" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersDiluted_90b895b8-5b6b-47cb-9ef4-b4f6b4cb1d3a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersDiluted"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_71d4ed56-7aba-4cd8-882d-758f6b2a4fea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersDiluted_90b895b8-5b6b-47cb-9ef4-b4f6b4cb1d3a" xlink:to="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_71d4ed56-7aba-4cd8-882d-758f6b2a4fea" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_800c62f8-1f0f-43e3-b7c7-f2633909b60a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingExpenses"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpense_60863029-edaf-438e-874e-6f2a99db9813" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GeneralAndAdministrativeExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_800c62f8-1f0f-43e3-b7c7-f2633909b60a" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpense_60863029-edaf-438e-874e-6f2a99db9813" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_f9038611-11d9-411f-82da-c02edb53a867" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_800c62f8-1f0f-43e3-b7c7-f2633909b60a" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_f9038611-11d9-411f-82da-c02edb53a867" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpense_0991a944-97d4-4241-9f7c-5450a4ba3498" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SellingAndMarketingExpense"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_800c62f8-1f0f-43e3-b7c7-f2633909b60a" xlink:to="loc_us-gaap_SellingAndMarketingExpense_0991a944-97d4-4241-9f7c-5450a4ba3498" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_5df368a3-18cf-48eb-b6a4-80a330b91f75" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_7509ae10-7912-4b6a-ae85-a1a6cad198d4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_5df368a3-18cf-48eb-b6a4-80a330b91f75" xlink:to="loc_us-gaap_OperatingIncomeLoss_7509ae10-7912-4b6a-ae85-a1a6cad198d4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_a79d0391-7910-43eb-b38e-793ad4650982" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_5df368a3-18cf-48eb-b6a4-80a330b91f75" xlink:to="loc_us-gaap_NonoperatingIncomeExpense_a79d0391-7910-43eb-b38e-793ad4650982" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_c4916fe2-8506-41be-aa64-5b8c2e693ec4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_b4c9027d-160b-4632-90d3-0b96494a23f6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GrossProfit"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_c4916fe2-8506-41be-aa64-5b8c2e693ec4" xlink:to="loc_us-gaap_GrossProfit_b4c9027d-160b-4632-90d3-0b96494a23f6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_5d5bf3d6-cdd1-4f75-825a-39a6a3e370db" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingExpenses"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_c4916fe2-8506-41be-aa64-5b8c2e693ec4" xlink:to="loc_us-gaap_OperatingExpenses_5d5bf3d6-cdd1-4f75-825a-39a6a3e370db" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_713316b3-8ac6-4920-9326-90f4085febf4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GrossProfit"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_053bbec5-51ac-45f3-a1dc-43e7f58be903" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_GrossProfit_713316b3-8ac6-4920-9326-90f4085febf4" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_053bbec5-51ac-45f3-a1dc-43e7f58be903" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_af67704a-27e3-48eb-806c-0cdeb6394ff3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_GrossProfit_713316b3-8ac6-4920-9326-90f4085febf4" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_af67704a-27e3-48eb-806c-0cdeb6394ff3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_1b2fa945-b0ed-4397-8bc6-2649b6705331" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseNonoperating_2db98484-4037-4937-afa5-277256e7086d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InterestExpenseNonoperating"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_1b2fa945-b0ed-4397-8bc6-2649b6705331" xlink:to="loc_us-gaap_InterestExpenseNonoperating_2db98484-4037-4937-afa5-277256e7086d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_832d72f9-2c82-4fcb-8739-30ca25e6bbd2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_1b2fa945-b0ed-4397-8bc6-2649b6705331" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_832d72f9-2c82-4fcb-8739-30ca25e6bbd2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentIncomeInterest_d0e3df83-4416-4bd3-87f4-75717e085698" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InvestmentIncomeInterest"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_1b2fa945-b0ed-4397-8bc6-2649b6705331" xlink:to="loc_us-gaap_InvestmentIncomeInterest_d0e3df83-4416-4bd3-87f4-75717e085698" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_c547df24-fc4d-4e36-8982-641b01508ccb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_41d785c1-4318-4f0a-ad9f-fca5908b3a9d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProfitLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_c547df24-fc4d-4e36-8982-641b01508ccb" xlink:to="loc_us-gaap_ProfitLoss_41d785c1-4318-4f0a-ad9f-fca5908b3a9d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_6aa8903a-9637-48e5-a2d8-11687d09ff84" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProfitLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_19691bb4-e283-4d55-b08e-5a21443346c9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ProfitLoss_6aa8903a-9637-48e5-a2d8-11687d09ff84" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_19691bb4-e283-4d55-b08e-5a21443346c9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_9c9e203e-3f55-41db-a87e-9c9b4aa5d1ad" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ProfitLoss_6aa8903a-9637-48e5-a2d8-11687d09ff84" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_9c9e203e-3f55-41db-a87e-9c9b4aa5d1ad" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_16ef0618-854b-4c38-ab2a-cb42ea672f5e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MarketableSecuritiesUnrealizedGainLoss_1507b46a-6ab8-4aa1-8cc3-51868a0d9c94" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_MarketableSecuritiesUnrealizedGainLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_16ef0618-854b-4c38-ab2a-cb42ea672f5e" xlink:to="loc_us-gaap_MarketableSecuritiesUnrealizedGainLoss_1507b46a-6ab8-4aa1-8cc3-51868a0d9c94" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax_47c52741-8009-405d-9c49-71d5013ed018" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_16ef0618-854b-4c38-ab2a-cb42ea672f5e" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax_47c52741-8009-405d-9c49-71d5013ed018" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_74d95ec1-929f-4def-b100-62c46ce93c1a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProfitLoss"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_16ef0618-854b-4c38-ab2a-cb42ea672f5e" xlink:to="loc_us-gaap_ProfitLoss_74d95ec1-929f-4def-b100-62c46ce93c1a" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/ConsolidatedStatementsofCashFlowsunaudited" xlink:type="simple" xlink:href="ctkb-20240930.xsd#ConsolidatedStatementsofCashFlowsunaudited"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/ConsolidatedStatementsofCashFlowsunaudited" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_IncreaseDecreaseInLegalSettlementLiabilities_6bd745f6-1eae-4b40-82d8-b69c35fb45d7" xlink:href="ctkb-20240930.xsd#ctkb_IncreaseDecreaseInLegalSettlementLiabilities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_ctkb_IncreaseDecreaseInLegalSettlementLiabilities_6bd745f6-1eae-4b40-82d8-b69c35fb45d7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_LossGainOnInvestmentsAccretionAndAmortizationNet_b9979590-4b73-4961-8f5e-d42d3e00ae4a" xlink:href="ctkb-20240930.xsd#ctkb_LossGainOnInvestmentsAccretionAndAmortizationNet"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_ctkb_LossGainOnInvestmentsAccretionAndAmortizationNet_b9979590-4b73-4961-8f5e-d42d3e00ae4a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_0598df4e-f780-4b58-924c-1c6a61be89c4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_0598df4e-f780-4b58-924c-1c6a61be89c4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_c0ef3fb1-df63-477d-ab7a-d40be0bb03f0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInDeferredRevenue"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_c0ef3fb1-df63-477d-ab7a-d40be0bb03f0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccruedLiabilities_c08599b8-6c5e-415b-b2e7-d614b5a43e4c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccruedLiabilities"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_IncreaseDecreaseInAccruedLiabilities_c08599b8-6c5e-415b-b2e7-d614b5a43e4c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_e41b620a-0e04-4924-b9a7-d8853d331780" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensation"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_ShareBasedCompensation_e41b620a-0e04-4924-b9a7-d8853d331780" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_3dfc8555-9c6d-4f61-a878-7e0fee9ae8f9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_3dfc8555-9c6d-4f61-a878-7e0fee9ae8f9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_62f6a581-9a17-4c74-a8fd-94aa88193e6b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
    <link:calculationArc order="8" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_62f6a581-9a17-4c74-a8fd-94aa88193e6b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_InterestExpensesForAccretionOfLegalSettlementLiabilities_5c98deba-235d-44fb-8fda-5173dbd9aec7" xlink:href="ctkb-20240930.xsd#ctkb_InterestExpensesForAccretionOfLegalSettlementLiabilities"/>
    <link:calculationArc order="9" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_ctkb_InterestExpensesForAccretionOfLegalSettlementLiabilities_5c98deba-235d-44fb-8fda-5173dbd9aec7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense_0c38303f-b88f-48a7-9ab0-20f81d630697" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense"/>
    <link:calculationArc order="10" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense_0c38303f-b88f-48a7-9ab0-20f81d630697" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryWriteDown_98a15502-7bf6-48c0-b9f7-8cc39c617829" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InventoryWriteDown"/>
    <link:calculationArc order="11" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_InventoryWriteDown_98a15502-7bf6-48c0-b9f7-8cc39c617829" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForDoubtfulAccounts_90ec81d1-3338-4f45-a9b1-aeba173b2aab" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProvisionForDoubtfulAccounts"/>
    <link:calculationArc order="12" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_ProvisionForDoubtfulAccounts_90ec81d1-3338-4f45-a9b1-aeba173b2aab" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_09e129b0-e9cd-4360-a3dd-2549e1f8ab94" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GainLossOnSaleOfPropertyPlantEquipment"/>
    <link:calculationArc order="13" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_09e129b0-e9cd-4360-a3dd-2549e1f8ab94" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_0a4fa401-eef6-43c0-9570-9ea80f3589f5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProfitLoss"/>
    <link:calculationArc order="14" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_ProfitLoss_0a4fa401-eef6-43c0-9570-9ea80f3589f5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOperatingLeaseLiability_42e91fed-1bc2-4698-a4ba-72657695ff9d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInOperatingLeaseLiability"/>
    <link:calculationArc order="15" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_IncreaseDecreaseInOperatingLeaseLiability_42e91fed-1bc2-4698-a4ba-72657695ff9d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInInventories_b629b970-33cd-44d5-874f-f3b8b16149d8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInInventories"/>
    <link:calculationArc order="16" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_IncreaseDecreaseInInventories_b629b970-33cd-44d5-874f-f3b8b16149d8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_fb29c9bc-8b74-4826-84c2-b9892a536ae1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable"/>
    <link:calculationArc order="17" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_add23e39-1ad1-466d-92d2-64c3b11a82c9" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_fb29c9bc-8b74-4826-84c2-b9892a536ae1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_ee91d792-f2dd-4541-a0f9-c5f0a9520d42" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_PurchasesOfMarketableSecurities_948ee591-fb70-496d-bb42-1516f52926bc" xlink:href="ctkb-20240930.xsd#ctkb_PurchasesOfMarketableSecurities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_ee91d792-f2dd-4541-a0f9-c5f0a9520d42" xlink:to="loc_ctkb_PurchasesOfMarketableSecurities_948ee591-fb70-496d-bb42-1516f52926bc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment_8fbca730-d164-4bb8-8158-2f5ba86a8a22" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_ee91d792-f2dd-4541-a0f9-c5f0a9520d42" xlink:to="loc_us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment_8fbca730-d164-4bb8-8158-2f5ba86a8a22" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireIntangibleAssets_67524b54-9a02-4c88-984f-c1f61f89cfea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsToAcquireIntangibleAssets"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_ee91d792-f2dd-4541-a0f9-c5f0a9520d42" xlink:to="loc_us-gaap_PaymentsToAcquireIntangibleAssets_67524b54-9a02-4c88-984f-c1f61f89cfea" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesGross_a71ca997-7b34-43bd-8584-28de82da59d6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsToAcquireBusinessesGross"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_ee91d792-f2dd-4541-a0f9-c5f0a9520d42" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesGross_a71ca997-7b34-43bd-8584-28de82da59d6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_PaymentForAdditionalInvestmentInCytekJapanNetOfCashAcquired_d2f48331-9083-4b4b-970e-5ab2fb0929f0" xlink:href="ctkb-20240930.xsd#ctkb_PaymentForAdditionalInvestmentInCytekJapanNetOfCashAcquired"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_ee91d792-f2dd-4541-a0f9-c5f0a9520d42" xlink:to="loc_ctkb_PaymentForAdditionalInvestmentInCytekJapanNetOfCashAcquired_d2f48331-9083-4b4b-970e-5ab2fb0929f0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleAndMaturityOfMarketableSecurities_18b0d578-7f89-4f95-b79f-d7affab5cf79" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromSaleAndMaturityOfMarketableSecurities"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_ee91d792-f2dd-4541-a0f9-c5f0a9520d42" xlink:to="loc_us-gaap_ProceedsFromSaleAndMaturityOfMarketableSecurities_18b0d578-7f89-4f95-b79f-d7affab5cf79" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_d4253608-0be3-445f-a073-3b350cdc8b05" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:calculationArc order="7" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_ee91d792-f2dd-4541-a0f9-c5f0a9520d42" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_d4253608-0be3-445f-a073-3b350cdc8b05" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_6831b48d-1e7b-4a87-9b0d-a0f3ac34efb2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_5e62c2c4-b26f-4b8b-9100-5680f3b654ef" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_6831b48d-1e7b-4a87-9b0d-a0f3ac34efb2" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_5e62c2c4-b26f-4b8b-9100-5680f3b654ef" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_ceb0deb9-3c03-46af-bfd9-d54e948945c7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_6831b48d-1e7b-4a87-9b0d-a0f3ac34efb2" xlink:to="loc_us-gaap_EffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_ceb0deb9-3c03-46af-bfd9-d54e948945c7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_4c4c4f5d-d405-47f8-8634-29dd2a7d6a6f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_6831b48d-1e7b-4a87-9b0d-a0f3ac34efb2" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_4c4c4f5d-d405-47f8-8634-29dd2a7d6a6f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_f615f8d1-4134-4a28-a642-d407c4d4cdea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_6831b48d-1e7b-4a87-9b0d-a0f3ac34efb2" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_f615f8d1-4134-4a28-a642-d407c4d4cdea" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_57ec4baf-e484-4592-aed1-bc748eea5b57" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromStockOptionsExercised_d59b09f3-028b-4f02-b64c-54b1b709ca79" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromStockOptionsExercised"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_57ec4baf-e484-4592-aed1-bc748eea5b57" xlink:to="loc_us-gaap_ProceedsFromStockOptionsExercised_d59b09f3-028b-4f02-b64c-54b1b709ca79" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromStockPlans_90a43552-dee0-4278-badc-326b83c57759" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromStockPlans"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_57ec4baf-e484-4592-aed1-bc748eea5b57" xlink:to="loc_us-gaap_ProceedsFromStockPlans_90a43552-dee0-4278-badc-326b83c57759" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromLinesOfCredit_1feeaea9-5a2e-4cb2-b27a-87802518bf65" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromLinesOfCredit"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_57ec4baf-e484-4592-aed1-bc748eea5b57" xlink:to="loc_us-gaap_ProceedsFromLinesOfCredit_1feeaea9-5a2e-4cb2-b27a-87802518bf65" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForRepurchaseOfEquity_403585b7-3754-43f5-b839-381b6a713329" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsForRepurchaseOfEquity"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_57ec4baf-e484-4592-aed1-bc748eea5b57" xlink:to="loc_us-gaap_PaymentsForRepurchaseOfEquity_403585b7-3754-43f5-b839-381b6a713329" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_PaymentsForTaxesRelatedToNetShareSettlementOfEquityAwards_eb83e81a-a89e-4837-a1a0-4219063a6f05" xlink:href="ctkb-20240930.xsd#ctkb_PaymentsForTaxesRelatedToNetShareSettlementOfEquityAwards"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_57ec4baf-e484-4592-aed1-bc748eea5b57" xlink:to="loc_ctkb_PaymentsForTaxesRelatedToNetShareSettlementOfEquityAwards_eb83e81a-a89e-4837-a1a0-4219063a6f05" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfDebt_8897d390-610a-4808-ad43-4c1dc247c599" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RepaymentsOfDebt"/>
    <link:calculationArc order="6" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_57ec4baf-e484-4592-aed1-bc748eea5b57" xlink:to="loc_us-gaap_RepaymentsOfDebt_8897d390-610a-4808-ad43-4c1dc247c599" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesSummaryofCashCashEquivalentsandRestrictedCashDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#BasisofPresentationandSummaryofSignificantAccountingPoliciesSummaryofCashCashEquivalentsandRestrictedCashDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesSummaryofCashCashEquivalentsandRestrictedCashDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_9b87fc0f-5825-41ff-b73a-82d956ada867" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentSecuritiesAtCarryingValue_cfabfed3-afd1-4fac-9a98-523621934d2b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_USGovernmentSecuritiesAtCarryingValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_9b87fc0f-5825-41ff-b73a-82d956ada867" xlink:to="loc_us-gaap_USGovernmentSecuritiesAtCarryingValue_cfabfed3-afd1-4fac-9a98-523621934d2b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCash_5f7f5c43-e069-4223-99b3-dab7338ec15d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCash"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_9b87fc0f-5825-41ff-b73a-82d956ada867" xlink:to="loc_us-gaap_RestrictedCash_5f7f5c43-e069-4223-99b3-dab7338ec15d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Cash_98d0e806-4f5c-46c8-ba44-36a9288bbd5c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Cash"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_9b87fc0f-5825-41ff-b73a-82d956ada867" xlink:to="loc_us-gaap_Cash_98d0e806-4f5c-46c8-ba44-36a9288bbd5c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsAtCarryingValue_95d2e6cb-858d-4523-9bdf-ba2cff77ca5d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_MoneyMarketFundsAtCarryingValue"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_9b87fc0f-5825-41ff-b73a-82d956ada867" xlink:to="loc_us-gaap_MoneyMarketFundsAtCarryingValue_95d2e6cb-858d-4523-9bdf-ba2cff77ca5d" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/RevenuefromContractswithCustomersScheduleofInformationAboutReceivablesCustomerDepositsandDeferredRevenueFromContractsWithCustomersDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#RevenuefromContractswithCustomersScheduleofInformationAboutReceivablesCustomerDepositsandDeferredRevenueFromContractsWithCustomersDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/RevenuefromContractswithCustomersScheduleofInformationAboutReceivablesCustomerDepositsandDeferredRevenueFromContractsWithCustomersDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiability_6230e170-ed21-44a5-adaa-5167d874d0bb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerLiability"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_49f30eba-267b-438a-9972-854d1ff79050" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ContractWithCustomerLiability_6230e170-ed21-44a5-adaa-5167d874d0bb" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_49f30eba-267b-438a-9972-854d1ff79050" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_CustomerDepositLiabilityCurrent_686a0857-5fdb-4472-8fb5-ce320755e41b" xlink:href="ctkb-20240930.xsd#ctkb_CustomerDepositLiabilityCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ContractWithCustomerLiability_6230e170-ed21-44a5-adaa-5167d874d0bb" xlink:to="loc_ctkb_CustomerDepositLiabilityCurrent_686a0857-5fdb-4472-8fb5-ce320755e41b" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofInventoryDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#BalanceSheetDetailsScheduleofComponentsofInventoryDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofInventoryDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryGross_1c0003e8-7194-4986-9064-aefe1434878c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InventoryGross"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryRawMaterials_96c42829-2a4c-4cfe-b9f1-d3c9054c4a55" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InventoryRawMaterials"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryGross_1c0003e8-7194-4986-9064-aefe1434878c" xlink:to="loc_us-gaap_InventoryRawMaterials_96c42829-2a4c-4cfe-b9f1-d3c9054c4a55" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryWorkInProcess_4bb293d5-93e5-43df-92ae-794b52135287" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InventoryWorkInProcess"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryGross_1c0003e8-7194-4986-9064-aefe1434878c" xlink:to="loc_us-gaap_InventoryWorkInProcess_4bb293d5-93e5-43df-92ae-794b52135287" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryFinishedGoods_6b85c45e-ebc6-473b-8880-01fb8e7887f1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InventoryFinishedGoods"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryGross_1c0003e8-7194-4986-9064-aefe1434878c" xlink:to="loc_us-gaap_InventoryFinishedGoods_6b85c45e-ebc6-473b-8880-01fb8e7887f1" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofPrepaidExpensesandOtherCurrentAssetsDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#BalanceSheetDetailsScheduleofComponentsofPrepaidExpensesandOtherCurrentAssetsDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofPrepaidExpensesandOtherCurrentAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_ff6d5670-7a21-45df-9179-ec81eecc6b67" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsCurrent_b1eb2dad-55e1-418a-b340-72800de60535" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherAssetsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_ff6d5670-7a21-45df-9179-ec81eecc6b67" xlink:to="loc_us-gaap_OtherAssetsCurrent_b1eb2dad-55e1-418a-b340-72800de60535" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_PrepaidInventory_6e0ca1ba-2292-4b0c-be3b-d578d4ffecea" xlink:href="ctkb-20240930.xsd#ctkb_PrepaidInventory"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_ff6d5670-7a21-45df-9179-ec81eecc6b67" xlink:to="loc_ctkb_PrepaidInventory_6e0ca1ba-2292-4b0c-be3b-d578d4ffecea" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidTaxes_89cc64d5-e76b-4c0d-b338-b74bd00defb4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidTaxes"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_ff6d5670-7a21-45df-9179-ec81eecc6b67" xlink:to="loc_us-gaap_PrepaidTaxes_89cc64d5-e76b-4c0d-b338-b74bd00defb4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidRent_db66e912-695d-41de-9d2e-38ea641d007e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidRent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_ff6d5670-7a21-45df-9179-ec81eecc6b67" xlink:to="loc_us-gaap_PrepaidRent_db66e912-695d-41de-9d2e-38ea641d007e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_TaxRefundReceivableAndPayable_b7bea360-5337-4053-8ce4-783d0abe954d" xlink:href="ctkb-20240930.xsd#ctkb_TaxRefundReceivableAndPayable"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_ff6d5670-7a21-45df-9179-ec81eecc6b67" xlink:to="loc_ctkb_TaxRefundReceivableAndPayable_b7bea360-5337-4053-8ce4-783d0abe954d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidInsurance_533a2369-1445-4f5b-94a2-a865b5fd2859" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidInsurance"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_ff6d5670-7a21-45df-9179-ec81eecc6b67" xlink:to="loc_us-gaap_PrepaidInsurance_533a2369-1445-4f5b-94a2-a865b5fd2859" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherPrepaidExpenseCurrent_7972d56a-db48-4e60-87d4-bf8248f23a99" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherPrepaidExpenseCurrent"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_ff6d5670-7a21-45df-9179-ec81eecc6b67" xlink:to="loc_us-gaap_OtherPrepaidExpenseCurrent_7972d56a-db48-4e60-87d4-bf8248f23a99" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/BalanceSheetDetailsSummaryofComponentsofAccruedExpensesDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#BalanceSheetDetailsSummaryofComponentsofAccruedExpensesDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/BalanceSheetDetailsSummaryofComponentsofAccruedExpensesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_7b5cdc88-03cb-4b17-b14e-e071797a8da2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedEmployeeBenefitsCurrent_630b472f-c3e5-486a-a6ea-5e98ee46168b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedEmployeeBenefitsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_7b5cdc88-03cb-4b17-b14e-e071797a8da2" xlink:to="loc_us-gaap_AccruedEmployeeBenefitsCurrent_630b472f-c3e5-486a-a6ea-5e98ee46168b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedProfessionalFeesCurrent_990dca44-cdb0-4fe9-8efd-a2b7bc67f699" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedProfessionalFeesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_7b5cdc88-03cb-4b17-b14e-e071797a8da2" xlink:to="loc_us-gaap_AccruedProfessionalFeesCurrent_990dca44-cdb0-4fe9-8efd-a2b7bc67f699" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_AccruedPurchases_83242dea-3a1c-4447-9015-c910f592b882" xlink:href="ctkb-20240930.xsd#ctkb_AccruedPurchases"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_7b5cdc88-03cb-4b17-b14e-e071797a8da2" xlink:to="loc_ctkb_AccruedPurchases_83242dea-3a1c-4447-9015-c910f592b882" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProductWarrantyAccrualClassifiedCurrent_ccdd1c4e-28bb-40c6-9647-425a869decd6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProductWarrantyAccrualClassifiedCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_7b5cdc88-03cb-4b17-b14e-e071797a8da2" xlink:to="loc_us-gaap_ProductWarrantyAccrualClassifiedCurrent_ccdd1c4e-28bb-40c6-9647-425a869decd6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAccruedLiabilitiesCurrent_2a3c5a8c-18a6-4870-9c14-b8ad18b85278" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherAccruedLiabilitiesCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_7b5cdc88-03cb-4b17-b14e-e071797a8da2" xlink:to="loc_us-gaap_OtherAccruedLiabilitiesCurrent_2a3c5a8c-18a6-4870-9c14-b8ad18b85278" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/BalanceSheetDetailsSummaryofComponentsofOtherCurrentLiabilitiesDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#BalanceSheetDetailsSummaryofComponentsofOtherCurrentLiabilitiesDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/BalanceSheetDetailsSummaryofComponentsofOtherCurrentLiabilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesCurrent_90421503-9526-4f86-8d08-300eab09faf6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_CustomerDepositLiabilityCurrent_c612392c-b638-4034-9daa-6fa262351392" xlink:href="ctkb-20240930.xsd#ctkb_CustomerDepositLiabilityCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherLiabilitiesCurrent_90421503-9526-4f86-8d08-300eab09faf6" xlink:to="loc_ctkb_CustomerDepositLiabilityCurrent_c612392c-b638-4034-9daa-6fa262351392" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_OtherCurrentLiabilitiesOther_3b2fef21-4915-4f9a-b6f4-df68e849137c" xlink:href="ctkb-20240930.xsd#ctkb_OtherCurrentLiabilitiesOther"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherLiabilitiesCurrent_90421503-9526-4f86-8d08-300eab09faf6" xlink:to="loc_ctkb_OtherCurrentLiabilitiesOther_3b2fef21-4915-4f9a-b6f4-df68e849137c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermConstructionLoanCurrent_24432d47-1d04-4a7c-b89f-4824eb5869b7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermConstructionLoanCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherLiabilitiesCurrent_90421503-9526-4f86-8d08-300eab09faf6" xlink:to="loc_us-gaap_LongTermConstructionLoanCurrent_24432d47-1d04-4a7c-b89f-4824eb5869b7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_db5fa6cb-813b-441b-a6c8-5c0d39ff2e1e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherLiabilitiesCurrent_90421503-9526-4f86-8d08-300eab09faf6" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_db5fa6cb-813b-441b-a6c8-5c0d39ff2e1e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedIncomeTaxesCurrent_ded1bc96-7daa-4e92-972c-4b5f72d59f31" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedIncomeTaxesCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherLiabilitiesCurrent_90421503-9526-4f86-8d08-300eab09faf6" xlink:to="loc_us-gaap_AccruedIncomeTaxesCurrent_ded1bc96-7daa-4e92-972c-4b5f72d59f31" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SalesAndExciseTaxPayableCurrent_377174cb-abfb-4f55-bd4e-4da387259365" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SalesAndExciseTaxPayableCurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherLiabilitiesCurrent_90421503-9526-4f86-8d08-300eab09faf6" xlink:to="loc_us-gaap_SalesAndExciseTaxPayableCurrent_377174cb-abfb-4f55-bd4e-4da387259365" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/PropertyandEquipmentNetSummaryofPropertyandEquipmentNetDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#PropertyandEquipmentNetSummaryofPropertyandEquipmentNetDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/PropertyandEquipmentNetSummaryofPropertyandEquipmentNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_234b3aa4-05b0-4deb-a1e6-681965eec9d2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_83ad0f40-28d9-4663-a49b-e4511327717e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_234b3aa4-05b0-4deb-a1e6-681965eec9d2" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_83ad0f40-28d9-4663-a49b-e4511327717e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_47eac81d-3f83-4e50-a2c3-1fdbaea25e30" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_234b3aa4-05b0-4deb-a1e6-681965eec9d2" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_47eac81d-3f83-4e50-a2c3-1fdbaea25e30" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/AcquisitionSummaryofAllocationofPurchasePricetotheAssetsAcquiredAsaPartoftheAcquisitionDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#AcquisitionSummaryofAllocationofPurchasePricetotheAssetsAcquiredAsaPartoftheAcquisitionDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/AcquisitionSummaryofAllocationofPurchasePricetotheAssetsAcquiredAsaPartoftheAcquisitionDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_001a1cfb-2250-4f96-917f-159cb925a6ea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill_74cfd493-7544-4c8e-8720-3f15e93d7ac8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_001a1cfb-2250-4f96-917f-159cb925a6ea" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill_74cfd493-7544-4c8e-8720-3f15e93d7ac8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesDeferredRevenue_a4527fa4-dc27-49cb-b81e-3e1873a8dee6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesDeferredRevenue"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_001a1cfb-2250-4f96-917f-159cb925a6ea" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesDeferredRevenue_a4527fa4-dc27-49cb-b81e-3e1873a8dee6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory_e05ddcec-0e12-4b5e-b6f4-8159d9e04b77" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_001a1cfb-2250-4f96-917f-159cb925a6ea" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory_e05ddcec-0e12-4b5e-b6f4-8159d9e04b77" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment_782b046e-04d4-48d6-86e4-48c65ef45820" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_001a1cfb-2250-4f96-917f-159cb925a6ea" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment_782b046e-04d4-48d6-86e4-48c65ef45820" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsPrepaidExpenseAndOtherAssets_fa6d2757-918f-4ed5-97bc-ba16c1b89ecf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsPrepaidExpenseAndOtherAssets"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_001a1cfb-2250-4f96-917f-159cb925a6ea" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsPrepaidExpenseAndOtherAssets_fa6d2757-918f-4ed5-97bc-ba16c1b89ecf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxAssets_508bf19b-ff9d-4f32-8082-b5716522dbb9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxAssets"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_001a1cfb-2250-4f96-917f-159cb925a6ea" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxAssets_508bf19b-ff9d-4f32-8082-b5716522dbb9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_d7e1b3e6-d5c6-47b8-9291-312f9078d7d2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Goodwill"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_001a1cfb-2250-4f96-917f-159cb925a6ea" xlink:to="loc_us-gaap_Goodwill_d7e1b3e6-d5c6-47b8-9291-312f9078d7d2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesOther_db46b08f-d291-42ee-a7b6-fc2b81291bd6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesOther"/>
    <link:calculationArc order="8" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_001a1cfb-2250-4f96-917f-159cb925a6ea" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesOther_db46b08f-d291-42ee-a7b6-fc2b81291bd6" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/GoodwillandIntangibleAssetsNetSummaryofIntangibleAssetsandGoodwillDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#GoodwillandIntangibleAssetsNetSummaryofIntangibleAssetsandGoodwillDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/GoodwillandIntangibleAssetsNetSummaryofIntangibleAssetsandGoodwillDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_b787d393-b505-45a0-aa1f-0ed3c1d46ff6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_86dad363-e67a-4c1a-bee5-20cdb582e93a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_b787d393-b505-45a0-aa1f-0ed3c1d46ff6" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_86dad363-e67a-4c1a-bee5-20cdb582e93a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_44249f93-66f4-4c06-921f-b4a1d7d59e9f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_b787d393-b505-45a0-aa1f-0ed3c1d46ff6" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_44249f93-66f4-4c06-921f-b4a1d7d59e9f" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/LegalSettlementLiabilityScheduleofLegalSettlementLiabilityDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#LegalSettlementLiabilityScheduleofLegalSettlementLiabilityDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/LegalSettlementLiabilityScheduleofLegalSettlementLiabilityDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_LegalSettlementLiability_6c74811a-0eb5-4cee-9eb0-8db1cabcc45c" xlink:href="ctkb-20240930.xsd#ctkb_LegalSettlementLiability"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_LegalSettlementLiabilityCurrent_81bc7085-c4ad-427d-93c4-8d8bc366caf8" xlink:href="ctkb-20240930.xsd#ctkb_LegalSettlementLiabilityCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_ctkb_LegalSettlementLiability_6c74811a-0eb5-4cee-9eb0-8db1cabcc45c" xlink:to="loc_ctkb_LegalSettlementLiabilityCurrent_81bc7085-c4ad-427d-93c4-8d8bc366caf8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_LegalSettlementLiabilityNonCurrent_0906d612-da47-45f2-8d20-effd6f10b2cc" xlink:href="ctkb-20240930.xsd#ctkb_LegalSettlementLiabilityNonCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_ctkb_LegalSettlementLiability_6c74811a-0eb5-4cee-9eb0-8db1cabcc45c" xlink:to="loc_ctkb_LegalSettlementLiabilityNonCurrent_0906d612-da47-45f2-8d20-effd6f10b2cc" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/LeaseScheduleofLeaseExpenseDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#LeaseScheduleofLeaseExpenseDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/LeaseScheduleofLeaseExpenseDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseCost_645268a7-998b-46b5-a9df-9061c79c5cae" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LeaseCost"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseCost_cb7a5a33-7240-48ae-813d-cd4f23f8ac83" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseCost"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LeaseCost_645268a7-998b-46b5-a9df-9061c79c5cae" xlink:to="loc_us-gaap_OperatingLeaseCost_cb7a5a33-7240-48ae-813d-cd4f23f8ac83" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermLeaseCost_fc509b5f-f817-46be-8930-5c7ba81cde00" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShortTermLeaseCost"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LeaseCost_645268a7-998b-46b5-a9df-9061c79c5cae" xlink:to="loc_us-gaap_ShortTermLeaseCost_fc509b5f-f817-46be-8930-5c7ba81cde00" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/LeaseScheduleofSupplementalBalanceSheetInformationRelatedtoLeasesDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#LeaseScheduleofSupplementalBalanceSheetInformationRelatedtoLeasesDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/LeaseScheduleofSupplementalBalanceSheetInformationRelatedtoLeasesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_d6780e2c-88eb-4db4-8e75-acf19304fa84" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_fe01ff40-f980-45ac-8ff6-e3ed06ddd254" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingLeaseLiability_d6780e2c-88eb-4db4-8e75-acf19304fa84" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_fe01ff40-f980-45ac-8ff6-e3ed06ddd254" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_f43a2513-ece4-4e6e-80a8-796193b18f72" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingLeaseLiability_d6780e2c-88eb-4db4-8e75-acf19304fa84" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_f43a2513-ece4-4e6e-80a8-796193b18f72" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a749b30b-5c10-442c-aff0-2725f18f4803" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_f3e78c91-6c05-4fc6-b27d-5e79cf408685" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a749b30b-5c10-442c-aff0-2725f18f4803" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_f3e78c91-6c05-4fc6-b27d-5e79cf408685" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_de67d456-3463-4c19-af6b-84c9bf066d36" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a749b30b-5c10-442c-aff0-2725f18f4803" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_de67d456-3463-4c19-af6b-84c9bf066d36" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_110de706-042d-480c-8747-bc1c3ff83775" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a749b30b-5c10-442c-aff0-2725f18f4803" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_110de706-042d-480c-8747-bc1c3ff83775" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_97695233-0146-4355-83c1-b7a3d840996d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a749b30b-5c10-442c-aff0-2725f18f4803" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_97695233-0146-4355-83c1-b7a3d840996d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_106edc91-d07a-4049-bc44-f2ac46bfb418" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a749b30b-5c10-442c-aff0-2725f18f4803" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_106edc91-d07a-4049-bc44-f2ac46bfb418" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_LesseeOperatingLeaseLiabilityToBePaidAfterYearFour_906d8b10-7213-4f35-b45a-8693cf8855aa" xlink:href="ctkb-20240930.xsd#ctkb_LesseeOperatingLeaseLiabilityToBePaidAfterYearFour"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_a749b30b-5c10-442c-aff0-2725f18f4803" xlink:to="loc_ctkb_LesseeOperatingLeaseLiabilityToBePaidAfterYearFour_906d8b10-7213-4f35-b45a-8693cf8855aa" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails_1" xlink:type="simple" xlink:href="ctkb-20240930.xsd#LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails_1"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_f8006c5a-3d80-4fa7-bc5d-8dab720feb4a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_7f6571e0-9280-47b3-aa12-270ab6df92dc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_f8006c5a-3d80-4fa7-bc5d-8dab720feb4a" xlink:to="loc_us-gaap_OperatingLeaseLiability_7f6571e0-9280-47b3-aa12-270ab6df92dc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_ea47288a-c03f-4458-8de8-0d1dd8e85d6d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_f8006c5a-3d80-4fa7-bc5d-8dab720feb4a" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_ea47288a-c03f-4458-8de8-0d1dd8e85d6d" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/CommitmentsandContingenciesScheduleoftheContractualObligationsforPurchaseObligationsDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#CommitmentsandContingenciesScheduleoftheContractualObligationsforPurchaseObligationsDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/CommitmentsandContingenciesScheduleoftheContractualObligationsforPurchaseObligationsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseObligation_5c976f76-5c5e-4dbb-9219-c9c7bc427617" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PurchaseObligation"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseObligationDueInNextTwelveMonths_ba8a5f3a-9529-478e-b950-3420c39ec274" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PurchaseObligationDueInNextTwelveMonths"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PurchaseObligation_5c976f76-5c5e-4dbb-9219-c9c7bc427617" xlink:to="loc_us-gaap_PurchaseObligationDueInNextTwelveMonths_ba8a5f3a-9529-478e-b950-3420c39ec274" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseObligationFutureMinimumPaymentsRemainderOfFiscalYear_8f0394e4-68b7-4ace-9eab-0f48955af82b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PurchaseObligationFutureMinimumPaymentsRemainderOfFiscalYear"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PurchaseObligation_5c976f76-5c5e-4dbb-9219-c9c7bc427617" xlink:to="loc_us-gaap_PurchaseObligationFutureMinimumPaymentsRemainderOfFiscalYear_8f0394e4-68b7-4ace-9eab-0f48955af82b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseObligationDueInThirdYear_20d634b8-8bf2-44b5-a620-0d3aae261f44" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PurchaseObligationDueInThirdYear"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PurchaseObligation_5c976f76-5c5e-4dbb-9219-c9c7bc427617" xlink:to="loc_us-gaap_PurchaseObligationDueInThirdYear_20d634b8-8bf2-44b5-a620-0d3aae261f44" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseObligationDueInSecondYear_33c097d2-9106-46c5-85ae-01aaf399f335" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PurchaseObligationDueInSecondYear"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PurchaseObligation_5c976f76-5c5e-4dbb-9219-c9c7bc427617" xlink:to="loc_us-gaap_PurchaseObligationDueInSecondYear_33c097d2-9106-46c5-85ae-01aaf399f335" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/NetIncomeLossPerShareComputationoftheBasicandDilutedNetIncomeAttributabletoCommonStockholdersPerShareDetails" xlink:type="simple" xlink:href="ctkb-20240930.xsd#NetIncomeLossPerShareComputationoftheBasicandDilutedNetIncomeAttributabletoCommonStockholdersPerShareDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/NetIncomeLossPerShareComputationoftheBasicandDilutedNetIncomeAttributabletoCommonStockholdersPerShareDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_83a03357-3615-4f61-97d4-679460f7c3f2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_39871979-211a-4a33-a9a2-dd250acb75e6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_83a03357-3615-4f61-97d4-679460f7c3f2" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_39871979-211a-4a33-a9a2-dd250acb75e6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncrementalCommonSharesAttributableToShareBasedPaymentArrangements_56087c7b-8bca-4123-b6fb-eba5bbbde7b8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncrementalCommonSharesAttributableToShareBasedPaymentArrangements"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_83a03357-3615-4f61-97d4-679460f7c3f2" xlink:to="loc_us-gaap_IncrementalCommonSharesAttributableToShareBasedPaymentArrangements_56087c7b-8bca-4123-b6fb-eba5bbbde7b8" xlink:type="arc"/>
  </link:calculationLink>
</link:linkbase>
