<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2025 Workiva-->
<!--r:1ce689f9-b377-4eb8-bf8f-58d48857c2a6,g:ebd70155-b4ab-45ce-a426-74b279e4b5d3-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://cytekbio.com/role/ConsolidatedBalanceSheets" xlink:type="simple" xlink:href="ctkb-20250331.xsd#ConsolidatedBalanceSheets"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/ConsolidatedBalanceSheets" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_8c3fcf6c-6533-4e62-8f71-8db8ff1a9d85" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableTradeCurrent_852a4db8-04ea-421c-ad95-31e471fc92f9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsPayableTradeCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_8c3fcf6c-6533-4e62-8f71-8db8ff1a9d85" xlink:to="loc_us-gaap_AccountsPayableTradeCurrent_852a4db8-04ea-421c-ad95-31e471fc92f9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SettlementLiabilitiesCurrent_8789a309-bf44-4dc4-8ce4-17202a81321f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SettlementLiabilitiesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_8c3fcf6c-6533-4e62-8f71-8db8ff1a9d85" xlink:to="loc_us-gaap_SettlementLiabilitiesCurrent_8789a309-bf44-4dc4-8ce4-17202a81321f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_903c761d-9547-4004-ae1f-970ec85d45e8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_8c3fcf6c-6533-4e62-8f71-8db8ff1a9d85" xlink:to="loc_us-gaap_AccruedLiabilitiesCurrent_903c761d-9547-4004-ae1f-970ec85d45e8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesCurrent_508b0583-3e5a-43b8-a5d5-9f5a00d35ea8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLiabilitiesCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_8c3fcf6c-6533-4e62-8f71-8db8ff1a9d85" xlink:to="loc_us-gaap_OtherLiabilitiesCurrent_508b0583-3e5a-43b8-a5d5-9f5a00d35ea8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredRevenueCurrent_15e44ec8-7297-4d07-8745-1a5fcbbe506c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredRevenueCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_8c3fcf6c-6533-4e62-8f71-8db8ff1a9d85" xlink:to="loc_us-gaap_DeferredRevenueCurrent_15e44ec8-7297-4d07-8745-1a5fcbbe506c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_316741cc-8bad-4374-9e4b-80cb473f1f95" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Liabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_d211c6a3-eb1b-4c33-b9a9-2d9c83a230be" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_316741cc-8bad-4374-9e4b-80cb473f1f95" xlink:to="loc_us-gaap_LiabilitiesCurrent_d211c6a3-eb1b-4c33-b9a9-2d9c83a230be" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LitigationReserveNoncurrent_8b40eed5-4fdb-45e3-9e98-9f9cba1849fd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LitigationReserveNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_316741cc-8bad-4374-9e4b-80cb473f1f95" xlink:to="loc_us-gaap_LitigationReserveNoncurrent_8b40eed5-4fdb-45e3-9e98-9f9cba1849fd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredRevenueNoncurrent_20b88a34-688d-4065-820a-1e674466eeab" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredRevenueNoncurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_316741cc-8bad-4374-9e4b-80cb473f1f95" xlink:to="loc_us-gaap_DeferredRevenueNoncurrent_20b88a34-688d-4065-820a-1e674466eeab" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_c4f06955-f423-43c7-8e05-3647c6ebedff" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_316741cc-8bad-4374-9e4b-80cb473f1f95" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_c4f06955-f423-43c7-8e05-3647c6ebedff" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_0ad503cb-3bd0-4f6f-ad89-e485868b9d6f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_316741cc-8bad-4374-9e4b-80cb473f1f95" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_0ad503cb-3bd0-4f6f-ad89-e485868b9d6f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_37ec66f0-9a1f-45f4-8c55-c8d692477c8f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_316741cc-8bad-4374-9e4b-80cb473f1f95" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_37ec66f0-9a1f-45f4-8c55-c8d692477c8f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_46648f6c-cc3e-43d0-816c-1282642a68fb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_68606d9b-ea65-4f9e-be05-594fe8fc2f24" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Liabilities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_46648f6c-cc3e-43d0-816c-1282642a68fb" xlink:to="loc_us-gaap_Liabilities_68606d9b-ea65-4f9e-be05-594fe8fc2f24" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingencies_e6ab26f1-8ce7-402b-9533-9e672f887698" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommitmentsAndContingencies"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_46648f6c-cc3e-43d0-816c-1282642a68fb" xlink:to="loc_us-gaap_CommitmentsAndContingencies_e6ab26f1-8ce7-402b-9533-9e672f887698" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_22d1fbe4-1634-406b-ab59-474ebd4401fa" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_46648f6c-cc3e-43d0-816c-1282642a68fb" xlink:to="loc_us-gaap_StockholdersEquity_22d1fbe4-1634-406b-ab59-474ebd4401fa" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_13666256-ca5f-4ad0-97a7-78368cb0830b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Assets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_6327fac1-2aeb-453e-903d-cfddfec6867b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_13666256-ca5f-4ad0-97a7-78368cb0830b" xlink:to="loc_us-gaap_AssetsCurrent_6327fac1-2aeb-453e-903d-cfddfec6867b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsDeferredIncome_50f94455-77a0-4c5f-9291-7d7f73ec19ee" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredTaxAssetsDeferredIncome"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_13666256-ca5f-4ad0-97a7-78368cb0830b" xlink:to="loc_us-gaap_DeferredTaxAssetsDeferredIncome_50f94455-77a0-4c5f-9291-7d7f73ec19ee" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_083fe5bd-b488-41e9-a530-4fa718cd2f5c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_13666256-ca5f-4ad0-97a7-78368cb0830b" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_083fe5bd-b488-41e9-a530-4fa718cd2f5c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_8f9200ce-f841-496d-984b-2fe6b8e35aa1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_13666256-ca5f-4ad0-97a7-78368cb0830b" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_8f9200ce-f841-496d-984b-2fe6b8e35aa1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_615ab2e4-fd71-4f77-9902-56162a905d06" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Goodwill"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_13666256-ca5f-4ad0-97a7-78368cb0830b" xlink:to="loc_us-gaap_Goodwill_615ab2e4-fd71-4f77-9902-56162a905d06" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_8bb67208-d5cf-4c9b-aa00-5f7bf4e16e8b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IntangibleAssetsNetExcludingGoodwill"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_13666256-ca5f-4ad0-97a7-78368cb0830b" xlink:to="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_8bb67208-d5cf-4c9b-aa00-5f7bf4e16e8b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_fe92eeaa-c53f-4096-bf7e-6345efd108e0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_13666256-ca5f-4ad0-97a7-78368cb0830b" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_fe92eeaa-c53f-4096-bf7e-6345efd108e0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_84e37f57-3f8b-4265-9f21-eac9b1da6ed0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_1007d21e-ea26-480d-8899-e26eaf77b191" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_84e37f57-3f8b-4265-9f21-eac9b1da6ed0" xlink:to="loc_us-gaap_CommonStockValue_1007d21e-ea26-480d-8899-e26eaf77b191" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_d75db693-c057-474e-be45-a1e67d23ddd1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_84e37f57-3f8b-4265-9f21-eac9b1da6ed0" xlink:to="loc_us-gaap_AdditionalPaidInCapital_d75db693-c057-474e-be45-a1e67d23ddd1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_c9ea9b84-b089-45aa-bff0-fde63b6046a2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_84e37f57-3f8b-4265-9f21-eac9b1da6ed0" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_c9ea9b84-b089-45aa-bff0-fde63b6046a2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_6830f8cf-d4eb-450e-bea2-fb01c2a3c000" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_84e37f57-3f8b-4265-9f21-eac9b1da6ed0" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_6830f8cf-d4eb-450e-bea2-fb01c2a3c000" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_4168956d-c402-4fcf-807a-ecdfdde03715" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_e6e9283e-35c4-4a83-844c-92ac5b182025" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_4168956d-c402-4fcf-807a-ecdfdde03715" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_e6e9283e-35c4-4a83-844c-92ac5b182025" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableNetCurrent_3b09c3ac-42c3-4b6e-bcae-9a9e5a076a19" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsReceivableNetCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_4168956d-c402-4fcf-807a-ecdfdde03715" xlink:to="loc_us-gaap_AccountsReceivableNetCurrent_3b09c3ac-42c3-4b6e-bcae-9a9e5a076a19" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashCurrent_0858b16f-86cb-4f96-b5c7-79187b34d5a7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCashCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_4168956d-c402-4fcf-807a-ecdfdde03715" xlink:to="loc_us-gaap_RestrictedCashCurrent_0858b16f-86cb-4f96-b5c7-79187b34d5a7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MarketableSecurities_7aeb7cce-81ed-412a-8cad-de285ddc0b35" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_MarketableSecurities"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_4168956d-c402-4fcf-807a-ecdfdde03715" xlink:to="loc_us-gaap_MarketableSecurities_7aeb7cce-81ed-412a-8cad-de285ddc0b35" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_0293f64c-cdf3-4c55-af3b-b1d60daff291" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InventoryNet"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_4168956d-c402-4fcf-807a-ecdfdde03715" xlink:to="loc_us-gaap_InventoryNet_0293f64c-cdf3-4c55-af3b-b1d60daff291" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_e4d0ad29-c325-4980-a39d-c271863f07d6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_4168956d-c402-4fcf-807a-ecdfdde03715" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_e4d0ad29-c325-4980-a39d-c271863f07d6" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/ConsolidatedStatementsofOperationsandComprehensiveLossunaudited" xlink:type="simple" xlink:href="ctkb-20250331.xsd#ConsolidatedStatementsofOperationsandComprehensiveLossunaudited"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/ConsolidatedStatementsofOperationsandComprehensiveLossunaudited" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_4370204f-bfd3-4a38-bceb-9676df2ff1c4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GrossProfit"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_2bd839b4-ac6c-4e40-bace-0c600f309297" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_GrossProfit_4370204f-bfd3-4a38-bceb-9676df2ff1c4" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_2bd839b4-ac6c-4e40-bace-0c600f309297" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_fbc2a37f-5d2f-4569-aab7-fade0fb73c05" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_GrossProfit_4370204f-bfd3-4a38-bceb-9676df2ff1c4" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_fbc2a37f-5d2f-4569-aab7-fade0fb73c05" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_e381afd5-9cd2-4db1-8c90-b39aa3a6c667" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_95ce1ac1-504d-459b-aec3-c40b595603d6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_e381afd5-9cd2-4db1-8c90-b39aa3a6c667" xlink:to="loc_us-gaap_OperatingIncomeLoss_95ce1ac1-504d-459b-aec3-c40b595603d6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_b7133910-da64-434f-ba5f-2bda009bcd21" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_e381afd5-9cd2-4db1-8c90-b39aa3a6c667" xlink:to="loc_us-gaap_NonoperatingIncomeExpense_b7133910-da64-434f-ba5f-2bda009bcd21" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_a7a69caa-c144-4446-a020-19ea69a693da" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProfitLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_3c18f16a-1acb-4270-858e-0ff69ddc91c8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ProfitLoss_a7a69caa-c144-4446-a020-19ea69a693da" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_3c18f16a-1acb-4270-858e-0ff69ddc91c8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_dad57e57-16ae-48ed-afab-d528a4203f1d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ProfitLoss_a7a69caa-c144-4446-a020-19ea69a693da" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_dad57e57-16ae-48ed-afab-d528a4203f1d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_00fdef48-95aa-48dd-a3db-8c3e266c4d4a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MarketableSecuritiesUnrealizedGainLoss_0f119f0f-124b-431f-bad1-333a2cca8d37" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_MarketableSecuritiesUnrealizedGainLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_00fdef48-95aa-48dd-a3db-8c3e266c4d4a" xlink:to="loc_us-gaap_MarketableSecuritiesUnrealizedGainLoss_0f119f0f-124b-431f-bad1-333a2cca8d37" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax_1e8b4e28-4b7b-43a9-b7a6-d8e46b93029e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_00fdef48-95aa-48dd-a3db-8c3e266c4d4a" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax_1e8b4e28-4b7b-43a9-b7a6-d8e46b93029e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_cba616aa-7e99-4b95-9f0c-63b83adb030b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProfitLoss"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_00fdef48-95aa-48dd-a3db-8c3e266c4d4a" xlink:to="loc_us-gaap_ProfitLoss_cba616aa-7e99-4b95-9f0c-63b83adb030b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_bcabd0fb-27ad-4acf-af86-23b0096b7914" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingExpenses"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpense_fd0c1cc7-ed94-4a25-b02b-9ea88520c535" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GeneralAndAdministrativeExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_bcabd0fb-27ad-4acf-af86-23b0096b7914" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpense_fd0c1cc7-ed94-4a25-b02b-9ea88520c535" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_397fc2b7-bd5b-438a-a17a-d315e5a30593" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_bcabd0fb-27ad-4acf-af86-23b0096b7914" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_397fc2b7-bd5b-438a-a17a-d315e5a30593" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpense_a60f33b9-7f61-47b0-87e7-023497fdeefb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SellingAndMarketingExpense"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_bcabd0fb-27ad-4acf-af86-23b0096b7914" xlink:to="loc_us-gaap_SellingAndMarketingExpense_a60f33b9-7f61-47b0-87e7-023497fdeefb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_58828847-61e9-48d3-9b0c-f6c388a03924" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseNonoperating_474ede5c-cc33-4a0b-bbac-1e4f3cfd5726" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InterestExpenseNonoperating"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_58828847-61e9-48d3-9b0c-f6c388a03924" xlink:to="loc_us-gaap_InterestExpenseNonoperating_474ede5c-cc33-4a0b-bbac-1e4f3cfd5726" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_631a57de-49f3-4c27-82b7-321894218c41" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_58828847-61e9-48d3-9b0c-f6c388a03924" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_631a57de-49f3-4c27-82b7-321894218c41" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentIncomeInterest_dcb857ce-c5c1-46e4-ad0b-f7ca896fb40d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InvestmentIncomeInterest"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NonoperatingIncomeExpense_58828847-61e9-48d3-9b0c-f6c388a03924" xlink:to="loc_us-gaap_InvestmentIncomeInterest_dcb857ce-c5c1-46e4-ad0b-f7ca896fb40d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersDiluted_5540e00a-58c5-461e-ba7e-3c0f11c4fd0b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersDiluted"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_01cf792c-2754-428c-a0ac-373a81f98ecd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProfitLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersDiluted_5540e00a-58c5-461e-ba7e-3c0f11c4fd0b" xlink:to="loc_us-gaap_ProfitLoss_01cf792c-2754-428c-a0ac-373a81f98ecd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_463dcff0-1c87-43bc-9208-2066b84cb37e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_cb83f03f-20c5-4c08-b69e-14349d3b3d45" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GrossProfit"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_463dcff0-1c87-43bc-9208-2066b84cb37e" xlink:to="loc_us-gaap_GrossProfit_cb83f03f-20c5-4c08-b69e-14349d3b3d45" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_029bee82-0128-4203-8a6b-559d15c400f8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingExpenses"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_463dcff0-1c87-43bc-9208-2066b84cb37e" xlink:to="loc_us-gaap_OperatingExpenses_029bee82-0128-4203-8a6b-559d15c400f8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_af5e5965-12f1-40f9-81a4-a680522a641e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_41acb559-90fc-4bba-9d7a-439c28db6053" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProfitLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_af5e5965-12f1-40f9-81a4-a680522a641e" xlink:to="loc_us-gaap_ProfitLoss_41acb559-90fc-4bba-9d7a-439c28db6053" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/ConsolidatedStatementsofCashFlowsunaudited" xlink:type="simple" xlink:href="ctkb-20250331.xsd#ConsolidatedStatementsofCashFlowsunaudited"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/ConsolidatedStatementsofCashFlowsunaudited" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_db4f7753-6b1f-4022-85a5-d4762a76ae2d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_PurchasesOfMarketableSecurities_29d08eb1-0e73-4584-b900-885189165f93" xlink:href="ctkb-20250331.xsd#ctkb_PurchasesOfMarketableSecurities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_db4f7753-6b1f-4022-85a5-d4762a76ae2d" xlink:to="loc_ctkb_PurchasesOfMarketableSecurities_29d08eb1-0e73-4584-b900-885189165f93" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleAndMaturityOfMarketableSecurities_3bf8ec0e-f851-4ac0-a21b-880e3da759a6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromSaleAndMaturityOfMarketableSecurities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_db4f7753-6b1f-4022-85a5-d4762a76ae2d" xlink:to="loc_us-gaap_ProceedsFromSaleAndMaturityOfMarketableSecurities_3bf8ec0e-f851-4ac0-a21b-880e3da759a6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment_c4e3b498-c3f5-403f-a2f8-df66e279bf8f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_db4f7753-6b1f-4022-85a5-d4762a76ae2d" xlink:to="loc_us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment_c4e3b498-c3f5-403f-a2f8-df66e279bf8f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_8c3444f2-9bbb-4519-9a02-a933c950c08a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_db4f7753-6b1f-4022-85a5-d4762a76ae2d" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_8c3444f2-9bbb-4519-9a02-a933c950c08a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireIntangibleAssets_f239e121-2dba-43e1-938b-cc13b5a3e5a2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsToAcquireIntangibleAssets"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_db4f7753-6b1f-4022-85a5-d4762a76ae2d" xlink:to="loc_us-gaap_PaymentsToAcquireIntangibleAssets_f239e121-2dba-43e1-938b-cc13b5a3e5a2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_7d9c2b28-69df-4b8d-b56a-931ea3f65c27" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromStockOptionsExercised_627cd94c-bd59-4a80-b1b0-527509d362cf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromStockOptionsExercised"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_7d9c2b28-69df-4b8d-b56a-931ea3f65c27" xlink:to="loc_us-gaap_ProceedsFromStockOptionsExercised_627cd94c-bd59-4a80-b1b0-527509d362cf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromLinesOfCredit_317af030-fb77-4a13-bd6b-8bdd48586f2d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProceedsFromLinesOfCredit"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_7d9c2b28-69df-4b8d-b56a-931ea3f65c27" xlink:to="loc_us-gaap_ProceedsFromLinesOfCredit_317af030-fb77-4a13-bd6b-8bdd48586f2d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_PaymentsForTaxesRelatedToNetShareSettlementOfEquityAwards_aee86869-1182-4ce9-aa85-e4210441ef15" xlink:href="ctkb-20250331.xsd#ctkb_PaymentsForTaxesRelatedToNetShareSettlementOfEquityAwards"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_7d9c2b28-69df-4b8d-b56a-931ea3f65c27" xlink:to="loc_ctkb_PaymentsForTaxesRelatedToNetShareSettlementOfEquityAwards_aee86869-1182-4ce9-aa85-e4210441ef15" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfDebt_e2090337-8089-4a7f-aaff-37e6f59e97dd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RepaymentsOfDebt"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_7d9c2b28-69df-4b8d-b56a-931ea3f65c27" xlink:to="loc_us-gaap_RepaymentsOfDebt_e2090337-8089-4a7f-aaff-37e6f59e97dd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_4cf9e30b-8b48-423b-b014-e76433e65522" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PaymentsForRepurchaseOfCommonStock"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_7d9c2b28-69df-4b8d-b56a-931ea3f65c27" xlink:to="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_4cf9e30b-8b48-423b-b014-e76433e65522" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7c949da5-9ab9-4481-9e54-cc6a7f387c9e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_0dfe8d27-7a40-4385-8337-da55b8e009c5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7c949da5-9ab9-4481-9e54-cc6a7f387c9e" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_0dfe8d27-7a40-4385-8337-da55b8e009c5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_9bfa6c17-c7d3-4528-8f24-ad66fecc362b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7c949da5-9ab9-4481-9e54-cc6a7f387c9e" xlink:to="loc_us-gaap_EffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_9bfa6c17-c7d3-4528-8f24-ad66fecc362b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_579a0519-df26-4d1b-b1b3-b43766dffcd6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7c949da5-9ab9-4481-9e54-cc6a7f387c9e" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_579a0519-df26-4d1b-b1b3-b43766dffcd6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_eeec4907-f79e-4342-809c-8b83283ecafe" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_7c949da5-9ab9-4481-9e54-cc6a7f387c9e" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_eeec4907-f79e-4342-809c-8b83283ecafe" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense_6db026f1-f05f-4862-b0e3-dd338b5d413d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense_6db026f1-f05f-4862-b0e3-dd338b5d413d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_196e5cea-e0c6-4543-a00d-aa8a930b00fc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_196e5cea-e0c6-4543-a00d-aa8a930b00fc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_5cf221ec-dd90-474e-ad73-450bb0e40605" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_5cf221ec-dd90-474e-ad73-450bb0e40605" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_0e992a07-024e-4dc3-9148-e359bcd05377" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_0e992a07-024e-4dc3-9148-e359bcd05377" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxExpenseBenefit_86a19ab7-7f40-479f-953e-b35e75235668" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredIncomeTaxExpenseBenefit"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_DeferredIncomeTaxExpenseBenefit_86a19ab7-7f40-479f-953e-b35e75235668" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_5d43a8b0-2e0a-43e9-a548-2b842506d0d8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProfitLoss"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_ProfitLoss_5d43a8b0-2e0a-43e9-a548-2b842506d0d8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_059545e7-3126-441e-8fc2-930d8da7e9ce" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInDeferredRevenue"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_059545e7-3126-441e-8fc2-930d8da7e9ce" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOperatingLeaseLiability_1e40a1ff-95c3-4bbe-b58d-fe8e9ea7dbdf" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInOperatingLeaseLiability"/>
    <link:calculationArc order="8" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_IncreaseDecreaseInOperatingLeaseLiability_1e40a1ff-95c3-4bbe-b58d-fe8e9ea7dbdf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_IncreaseDecreaseInLegalSettlementLiabilities_88c3854f-3441-482e-90c5-50a5e369fe76" xlink:href="ctkb-20250331.xsd#ctkb_IncreaseDecreaseInLegalSettlementLiabilities"/>
    <link:calculationArc order="9" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_ctkb_IncreaseDecreaseInLegalSettlementLiabilities_88c3854f-3441-482e-90c5-50a5e369fe76" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_1c64128c-00bf-4c41-9b2c-c26bc489a8d5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets"/>
    <link:calculationArc order="10" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_1c64128c-00bf-4c41-9b2c-c26bc489a8d5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForDoubtfulAccounts_1a947113-bd35-41f9-a8bb-67374c96532f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProvisionForDoubtfulAccounts"/>
    <link:calculationArc order="11" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_ProvisionForDoubtfulAccounts_1a947113-bd35-41f9-a8bb-67374c96532f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_e7af384c-6a95-4a46-8d52-a204ec247b67" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShareBasedCompensation"/>
    <link:calculationArc order="12" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_ShareBasedCompensation_e7af384c-6a95-4a46-8d52-a204ec247b67" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_LossGainOnInvestmentsAccretionAndAmortizationNet_10cf8071-fa4b-492a-9ea1-d5e3d1737f62" xlink:href="ctkb-20250331.xsd#ctkb_LossGainOnInvestmentsAccretionAndAmortizationNet"/>
    <link:calculationArc order="13" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_ctkb_LossGainOnInvestmentsAccretionAndAmortizationNet_10cf8071-fa4b-492a-9ea1-d5e3d1737f62" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_InterestExpensesForAccretionOfLegalSettlementLiabilities_0362cecc-8275-4687-96b6-0d5e64b362e2" xlink:href="ctkb-20250331.xsd#ctkb_InterestExpensesForAccretionOfLegalSettlementLiabilities"/>
    <link:calculationArc order="14" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_ctkb_InterestExpensesForAccretionOfLegalSettlementLiabilities_0362cecc-8275-4687-96b6-0d5e64b362e2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryWriteDown_ccddf57b-c8ac-4948-b7d8-bc790dbc0de4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InventoryWriteDown"/>
    <link:calculationArc order="15" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_InventoryWriteDown_ccddf57b-c8ac-4948-b7d8-bc790dbc0de4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccruedLiabilities_c029ceab-5104-4b4e-8fd7-c32dc8d36072" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInAccruedLiabilities"/>
    <link:calculationArc order="16" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_IncreaseDecreaseInAccruedLiabilities_c029ceab-5104-4b4e-8fd7-c32dc8d36072" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_d31e4856-ba8d-45f8-a895-99e77569ba13" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GainLossOnSaleOfPropertyPlantEquipment"/>
    <link:calculationArc order="17" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_GainLossOnSaleOfPropertyPlantEquipment_d31e4856-ba8d-45f8-a895-99e77569ba13" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInInventories_47b406c3-1260-409d-a9f0-67afc2b49474" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInInventories"/>
    <link:calculationArc order="18" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_d8402d49-62d8-41fe-a1d5-f5de6f859969" xlink:to="loc_us-gaap_IncreaseDecreaseInInventories_47b406c3-1260-409d-a9f0-67afc2b49474" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesScheduleofCashCashEquivalentsandRestrictedCashDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#BasisofPresentationandSummaryofSignificantAccountingPoliciesScheduleofCashCashEquivalentsandRestrictedCashDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesScheduleofCashCashEquivalentsandRestrictedCashDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_d213fee6-50e0-47c0-8070-ab52246915af" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Cash_167b9397-3ab5-4ce3-bed1-c1673048a358" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Cash"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_d213fee6-50e0-47c0-8070-ab52246915af" xlink:to="loc_us-gaap_Cash_167b9397-3ab5-4ce3-bed1-c1673048a358" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USGovernmentSecuritiesAtCarryingValue_84a356ed-d0b5-4021-a3ad-3cce43231a5c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_USGovernmentSecuritiesAtCarryingValue"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_d213fee6-50e0-47c0-8070-ab52246915af" xlink:to="loc_us-gaap_USGovernmentSecuritiesAtCarryingValue_84a356ed-d0b5-4021-a3ad-3cce43231a5c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCash_dc5b757f-fac4-4a81-a7d2-787442a38268" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RestrictedCash"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_d213fee6-50e0-47c0-8070-ab52246915af" xlink:to="loc_us-gaap_RestrictedCash_dc5b757f-fac4-4a81-a7d2-787442a38268" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsAtCarryingValue_778499ab-987c-467e-9bff-c2b8e86b90ea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_MoneyMarketFundsAtCarryingValue"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_d213fee6-50e0-47c0-8070-ab52246915af" xlink:to="loc_us-gaap_MoneyMarketFundsAtCarryingValue_778499ab-987c-467e-9bff-c2b8e86b90ea" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/RevenuefromContractswithCustomersScheduleofInformationAboutReceivablesDeferredRevenueFromContractsWithCustomersandCustomerDepositsDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#RevenuefromContractswithCustomersScheduleofInformationAboutReceivablesDeferredRevenueFromContractsWithCustomersandCustomerDepositsDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/RevenuefromContractswithCustomersScheduleofInformationAboutReceivablesDeferredRevenueFromContractsWithCustomersandCustomerDepositsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiability_88213f3d-e221-4e14-9d7a-bdf79ee72547" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerLiability"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_132d1c00-3bf0-4ef6-b805-dc7c1511d355" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerLiabilityNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ContractWithCustomerLiability_88213f3d-e221-4e14-9d7a-bdf79ee72547" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_132d1c00-3bf0-4ef6-b805-dc7c1511d355" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_CustomerDepositLiabilityCurrent_d76d08bd-03e4-425f-a587-1c5f13b8dda3" xlink:href="ctkb-20250331.xsd#ctkb_CustomerDepositLiabilityCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ContractWithCustomerLiability_88213f3d-e221-4e14-9d7a-bdf79ee72547" xlink:to="loc_ctkb_CustomerDepositLiabilityCurrent_d76d08bd-03e4-425f-a587-1c5f13b8dda3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_4304982d-4458-4663-b17c-40fb0722e240" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ContractWithCustomerLiability_88213f3d-e221-4e14-9d7a-bdf79ee72547" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_4304982d-4458-4663-b17c-40fb0722e240" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofInventoryDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#BalanceSheetDetailsScheduleofComponentsofInventoryDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofInventoryDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryGross_06bd9e23-1173-4c58-ac35-b09cea1bd660" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InventoryGross"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryRawMaterials_613dddc3-422e-4a0c-be89-59612add8fe7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InventoryRawMaterials"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryGross_06bd9e23-1173-4c58-ac35-b09cea1bd660" xlink:to="loc_us-gaap_InventoryRawMaterials_613dddc3-422e-4a0c-be89-59612add8fe7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryWorkInProcess_24eda426-2ce2-4244-8e12-137d66038784" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InventoryWorkInProcess"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryGross_06bd9e23-1173-4c58-ac35-b09cea1bd660" xlink:to="loc_us-gaap_InventoryWorkInProcess_24eda426-2ce2-4244-8e12-137d66038784" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryFinishedGoods_2698d0a0-d2ef-4ffe-91f0-790247e4126a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InventoryFinishedGoods"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryGross_06bd9e23-1173-4c58-ac35-b09cea1bd660" xlink:to="loc_us-gaap_InventoryFinishedGoods_2698d0a0-d2ef-4ffe-91f0-790247e4126a" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofPrepaidExpensesandOtherCurrentAssetsDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#BalanceSheetDetailsScheduleofComponentsofPrepaidExpensesandOtherCurrentAssetsDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofPrepaidExpensesandOtherCurrentAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_5027d1db-79fa-44b5-bfe7-4fa9a985ea89" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsCurrent_24195c3b-d2ab-4eb6-a5e1-9ebda8883afa" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherAssetsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_5027d1db-79fa-44b5-bfe7-4fa9a985ea89" xlink:to="loc_us-gaap_OtherAssetsCurrent_24195c3b-d2ab-4eb6-a5e1-9ebda8883afa" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_PrepaidInventory_55cc8b55-0e33-47d2-8379-64d5525126a6" xlink:href="ctkb-20250331.xsd#ctkb_PrepaidInventory"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_5027d1db-79fa-44b5-bfe7-4fa9a985ea89" xlink:to="loc_ctkb_PrepaidInventory_55cc8b55-0e33-47d2-8379-64d5525126a6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidTaxes_78af26fa-ef68-4abc-8546-1e6e69f09997" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidTaxes"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_5027d1db-79fa-44b5-bfe7-4fa9a985ea89" xlink:to="loc_us-gaap_PrepaidTaxes_78af26fa-ef68-4abc-8546-1e6e69f09997" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidRent_617ed912-3800-481e-be22-c4def38901b7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidRent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_5027d1db-79fa-44b5-bfe7-4fa9a985ea89" xlink:to="loc_us-gaap_PrepaidRent_617ed912-3800-481e-be22-c4def38901b7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_TaxRefundReceivableAndPayable_0817c8f8-b801-492f-beec-6225551f5d17" xlink:href="ctkb-20250331.xsd#ctkb_TaxRefundReceivableAndPayable"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_5027d1db-79fa-44b5-bfe7-4fa9a985ea89" xlink:to="loc_ctkb_TaxRefundReceivableAndPayable_0817c8f8-b801-492f-beec-6225551f5d17" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidInsurance_5b19ec82-ca8a-4959-8b0e-3b19ce53964b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PrepaidInsurance"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_5027d1db-79fa-44b5-bfe7-4fa9a985ea89" xlink:to="loc_us-gaap_PrepaidInsurance_5b19ec82-ca8a-4959-8b0e-3b19ce53964b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherPrepaidExpenseCurrent_f51110cb-6caa-4165-8197-08b2188dedf9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherPrepaidExpenseCurrent"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_5027d1db-79fa-44b5-bfe7-4fa9a985ea89" xlink:to="loc_us-gaap_OtherPrepaidExpenseCurrent_f51110cb-6caa-4165-8197-08b2188dedf9" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofAccruedExpensesDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#BalanceSheetDetailsScheduleofComponentsofAccruedExpensesDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofAccruedExpensesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_c1b5c503-a488-457f-9b1d-4f5c84522a4d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedEmployeeBenefitsCurrent_52276ba4-dddc-43ee-9fcb-5a918d713d7f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedEmployeeBenefitsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_c1b5c503-a488-457f-9b1d-4f5c84522a4d" xlink:to="loc_us-gaap_AccruedEmployeeBenefitsCurrent_52276ba4-dddc-43ee-9fcb-5a918d713d7f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedProfessionalFeesCurrent_37daf95e-000b-4c68-998d-4a983fb0dca1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedProfessionalFeesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_c1b5c503-a488-457f-9b1d-4f5c84522a4d" xlink:to="loc_us-gaap_AccruedProfessionalFeesCurrent_37daf95e-000b-4c68-998d-4a983fb0dca1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_AccruedPurchases_5d82419f-bcca-4605-875d-eb2d710e5808" xlink:href="ctkb-20250331.xsd#ctkb_AccruedPurchases"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_c1b5c503-a488-457f-9b1d-4f5c84522a4d" xlink:to="loc_ctkb_AccruedPurchases_5d82419f-bcca-4605-875d-eb2d710e5808" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProductWarrantyAccrualClassifiedCurrent_cb4273aa-d1fd-4b37-883a-6744157f41b4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ProductWarrantyAccrualClassifiedCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_c1b5c503-a488-457f-9b1d-4f5c84522a4d" xlink:to="loc_us-gaap_ProductWarrantyAccrualClassifiedCurrent_cb4273aa-d1fd-4b37-883a-6744157f41b4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAccruedLiabilitiesCurrent_e7b44837-e4e3-437a-9c06-94e2450036a6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherAccruedLiabilitiesCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent_c1b5c503-a488-457f-9b1d-4f5c84522a4d" xlink:to="loc_us-gaap_OtherAccruedLiabilitiesCurrent_e7b44837-e4e3-437a-9c06-94e2450036a6" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofOtherCurrentLiabilitiesDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#BalanceSheetDetailsScheduleofComponentsofOtherCurrentLiabilitiesDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofOtherCurrentLiabilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesCurrent_136aaa70-9400-4298-b708-5c8736d292bb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherLiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_CustomerDepositLiabilityCurrent_d96db9d0-ca5b-4ac1-ab66-1bea7a4378e2" xlink:href="ctkb-20250331.xsd#ctkb_CustomerDepositLiabilityCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherLiabilitiesCurrent_136aaa70-9400-4298-b708-5c8736d292bb" xlink:to="loc_ctkb_CustomerDepositLiabilityCurrent_d96db9d0-ca5b-4ac1-ab66-1bea7a4378e2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_OtherCurrentLiabilitiesOther_86251e84-8f53-41aa-8267-a59fee92e690" xlink:href="ctkb-20250331.xsd#ctkb_OtherCurrentLiabilitiesOther"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherLiabilitiesCurrent_136aaa70-9400-4298-b708-5c8736d292bb" xlink:to="loc_ctkb_OtherCurrentLiabilitiesOther_86251e84-8f53-41aa-8267-a59fee92e690" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermConstructionLoanCurrent_ab171282-e721-4a40-97ea-647437738f29" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermConstructionLoanCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherLiabilitiesCurrent_136aaa70-9400-4298-b708-5c8736d292bb" xlink:to="loc_us-gaap_LongTermConstructionLoanCurrent_ab171282-e721-4a40-97ea-647437738f29" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_441844e2-9dd4-4d76-8057-7d6f876394e8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherLiabilitiesCurrent_136aaa70-9400-4298-b708-5c8736d292bb" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_441844e2-9dd4-4d76-8057-7d6f876394e8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedIncomeTaxesCurrent_ed39f216-2aad-45b4-b7cf-ed1de630526f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccruedIncomeTaxesCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherLiabilitiesCurrent_136aaa70-9400-4298-b708-5c8736d292bb" xlink:to="loc_us-gaap_AccruedIncomeTaxesCurrent_ed39f216-2aad-45b4-b7cf-ed1de630526f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SalesAndExciseTaxPayableCurrent_dcf3d3ce-1c18-4130-a9f9-316552f580ba" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SalesAndExciseTaxPayableCurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherLiabilitiesCurrent_136aaa70-9400-4298-b708-5c8736d292bb" xlink:to="loc_us-gaap_SalesAndExciseTaxPayableCurrent_dcf3d3ce-1c18-4130-a9f9-316552f580ba" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/FairValueofFinancialInstrumentsScheduleofFinancialInstrumentsMeasuredonRecurringBasisDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#FairValueofFinancialInstrumentsScheduleofFinancialInstrumentsMeasuredonRecurringBasisDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/FairValueofFinancialInstrumentsScheduleofFinancialInstrumentsMeasuredonRecurringBasisDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_e6dd3d0c-375e-4788-b4d9-c00578a37e76" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MarketableSecurities_848bf9c7-96a8-49eb-88f1-282a4caa8e70" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_MarketableSecurities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsFairValueDisclosure_e6dd3d0c-375e-4788-b4d9-c00578a37e76" xlink:to="loc_us-gaap_MarketableSecurities_848bf9c7-96a8-49eb-88f1-282a4caa8e70" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_5f316411-8c12-4e6d-a095-02120c4d2e50" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsFairValueDisclosure_e6dd3d0c-375e-4788-b4d9-c00578a37e76" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_5f316411-8c12-4e6d-a095-02120c4d2e50" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/InvestmentsScheduleofInvestmentsinAvailableforSaleSecuritiesDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#InvestmentsScheduleofInvestmentsinAvailableforSaleSecuritiesDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/InvestmentsScheduleofInvestmentsinAvailableforSaleSecuritiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_000e4236-4d6a-4fcb-bc0f-e03fe362cd5d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_71f0caa2-db7b-4522-90f0-56f1b6a9c427" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_000e4236-4d6a-4fcb-bc0f-e03fe362cd5d" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_71f0caa2-db7b-4522-90f0-56f1b6a9c427" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_68e78bdd-bbfe-4328-8030-dd3061a79302" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_000e4236-4d6a-4fcb-bc0f-e03fe362cd5d" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_68e78bdd-bbfe-4328-8030-dd3061a79302" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_490660e8-daa9-46e3-a03a-b7fdb441eaab" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecurities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AvailableForSaleDebtSecuritiesAmortizedCostBasis_000e4236-4d6a-4fcb-bc0f-e03fe362cd5d" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecurities_490660e8-daa9-46e3-a03a-b7fdb441eaab" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/PropertyandEquipmentNetScheduleofComponentsofPropertyandEquipmentNetDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#PropertyandEquipmentNetScheduleofComponentsofPropertyandEquipmentNetDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/PropertyandEquipmentNetScheduleofComponentsofPropertyandEquipmentNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_0ba040cf-3048-42c5-880f-ac2b2302aaae" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_8894635e-3dc3-4f41-8ab7-d8058d470c0f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_0ba040cf-3048-42c5-880f-ac2b2302aaae" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_8894635e-3dc3-4f41-8ab7-d8058d470c0f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_8b4243ad-76af-4ef1-afa5-8da75f6d9d57" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_0ba040cf-3048-42c5-880f-ac2b2302aaae" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_8b4243ad-76af-4ef1-afa5-8da75f6d9d57" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/AcquisitionScheduleofEstimatedFairValueofAssetsAcquiredandLiabilitiesAssumedDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#AcquisitionScheduleofEstimatedFairValueofAssetsAcquiredandLiabilitiesAssumedDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/AcquisitionScheduleofEstimatedFairValueofAssetsAcquiredandLiabilitiesAssumedDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_5f7787b1-26b9-43df-bddb-e4bb839489dd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill_3155b25d-0d18-4993-a879-fb15d8264b31" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_5f7787b1-26b9-43df-bddb-e4bb839489dd" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill_3155b25d-0d18-4993-a879-fb15d8264b31" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesDeferredRevenue_8b347b29-bb8d-4703-b666-8233d0bea419" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesDeferredRevenue"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_5f7787b1-26b9-43df-bddb-e4bb839489dd" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesDeferredRevenue_8b347b29-bb8d-4703-b666-8233d0bea419" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory_fd18eecc-d194-42cc-b3ee-6e3c3f900ea5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_5f7787b1-26b9-43df-bddb-e4bb839489dd" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory_fd18eecc-d194-42cc-b3ee-6e3c3f900ea5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment_f3afaa2d-dbc5-4420-a9ae-26a10a0d0094" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_5f7787b1-26b9-43df-bddb-e4bb839489dd" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment_f3afaa2d-dbc5-4420-a9ae-26a10a0d0094" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsPrepaidExpenseAndOtherAssets_4598c8cc-0271-4fca-ad88-9a3a11dac3a9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsPrepaidExpenseAndOtherAssets"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_5f7787b1-26b9-43df-bddb-e4bb839489dd" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsPrepaidExpenseAndOtherAssets_4598c8cc-0271-4fca-ad88-9a3a11dac3a9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxAssets_b1f7237e-5d59-48a4-91d3-1f353a7c0da9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxAssets"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_5f7787b1-26b9-43df-bddb-e4bb839489dd" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxAssets_b1f7237e-5d59-48a4-91d3-1f353a7c0da9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_8130ba26-fb4d-40c6-b41f-ec745005e4a6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Goodwill"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_5f7787b1-26b9-43df-bddb-e4bb839489dd" xlink:to="loc_us-gaap_Goodwill_8130ba26-fb4d-40c6-b41f-ec745005e4a6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesOther_dd76f601-80c1-449f-916a-9e4bcf0b4e17" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesOther"/>
    <link:calculationArc order="8" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_5f7787b1-26b9-43df-bddb-e4bb839489dd" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesOther_dd76f601-80c1-449f-916a-9e4bcf0b4e17" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/GoodwillandIntangibleAssetsNetScheduleofComponentsofIntangibleAssetsNetDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#GoodwillandIntangibleAssetsNetScheduleofComponentsofIntangibleAssetsNetDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/GoodwillandIntangibleAssetsNetScheduleofComponentsofIntangibleAssetsNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_86957289-fb4e-451d-b9ac-5833a58d04fc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_ac7d3d25-def2-4981-bb3c-bca27f4b19ef" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_86957289-fb4e-451d-b9ac-5833a58d04fc" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_ac7d3d25-def2-4981-bb3c-bca27f4b19ef" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_cf493879-fc78-415a-8f52-aa10a1b1a47c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_86957289-fb4e-451d-b9ac-5833a58d04fc" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_cf493879-fc78-415a-8f52-aa10a1b1a47c" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/LegalSettlementLiabilityScheduleofLegalSettlementLiabilityDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#LegalSettlementLiabilityScheduleofLegalSettlementLiabilityDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/LegalSettlementLiabilityScheduleofLegalSettlementLiabilityDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_LegalSettlementLiability_c771fb68-b9e7-4a80-bf35-9a9b4349e241" xlink:href="ctkb-20250331.xsd#ctkb_LegalSettlementLiability"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_LegalSettlementLiabilityCurrent_398bae59-2ef0-4c89-a9c0-e7d20f4f02a8" xlink:href="ctkb-20250331.xsd#ctkb_LegalSettlementLiabilityCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_ctkb_LegalSettlementLiability_c771fb68-b9e7-4a80-bf35-9a9b4349e241" xlink:to="loc_ctkb_LegalSettlementLiabilityCurrent_398bae59-2ef0-4c89-a9c0-e7d20f4f02a8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_LegalSettlementLiabilityNonCurrent_3a3f52b6-1dc3-4d1f-b558-edd134b4e239" xlink:href="ctkb-20250331.xsd#ctkb_LegalSettlementLiabilityNonCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_ctkb_LegalSettlementLiability_c771fb68-b9e7-4a80-bf35-9a9b4349e241" xlink:to="loc_ctkb_LegalSettlementLiabilityNonCurrent_3a3f52b6-1dc3-4d1f-b558-edd134b4e239" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/LeaseScheduleofComponentsofLeaseExpenseDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#LeaseScheduleofComponentsofLeaseExpenseDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/LeaseScheduleofComponentsofLeaseExpenseDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseCost_57a22c50-059d-43cb-b49f-27e2648e647a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LeaseCost"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseCost_9bf7d8d9-aa90-437b-97f4-01190f678f1c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseCost"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LeaseCost_57a22c50-059d-43cb-b49f-27e2648e647a" xlink:to="loc_us-gaap_OperatingLeaseCost_9bf7d8d9-aa90-437b-97f4-01190f678f1c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermLeaseCost_10429886-9a56-42a2-bf70-66dd2eec44bc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShortTermLeaseCost"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LeaseCost_57a22c50-059d-43cb-b49f-27e2648e647a" xlink:to="loc_us-gaap_ShortTermLeaseCost_10429886-9a56-42a2-bf70-66dd2eec44bc" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/LeaseScheduleofSupplementalBalanceSheetInformationRelatedtoLeasesDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#LeaseScheduleofSupplementalBalanceSheetInformationRelatedtoLeasesDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/LeaseScheduleofSupplementalBalanceSheetInformationRelatedtoLeasesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_52b3e0c8-ecd3-43f0-add3-fec8940d88d7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_427fd145-0765-4295-a6f7-c9c6d45d1d9b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingLeaseLiability_52b3e0c8-ecd3-43f0-add3-fec8940d88d7" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_427fd145-0765-4295-a6f7-c9c6d45d1d9b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_ef560345-f864-4ceb-94af-8a1339e20fc9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingLeaseLiability_52b3e0c8-ecd3-43f0-add3-fec8940d88d7" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_ef560345-f864-4ceb-94af-8a1339e20fc9" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_cecea64a-fedb-4a46-888b-c365f6190ac5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_b630168f-3c0f-4055-9fa5-a09e7a229d8a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_cecea64a-fedb-4a46-888b-c365f6190ac5" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_b630168f-3c0f-4055-9fa5-a09e7a229d8a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_504ab736-82d3-4d31-9bba-b270197ee0af" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_cecea64a-fedb-4a46-888b-c365f6190ac5" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_504ab736-82d3-4d31-9bba-b270197ee0af" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_8869361b-601e-4585-be5b-954b362e6464" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_cecea64a-fedb-4a46-888b-c365f6190ac5" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_8869361b-601e-4585-be5b-954b362e6464" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_889782ef-aadc-42cc-ac83-e649da5fcb68" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_cecea64a-fedb-4a46-888b-c365f6190ac5" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_889782ef-aadc-42cc-ac83-e649da5fcb68" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_2a4baa0c-d400-496c-872d-dc48305fc690" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_cecea64a-fedb-4a46-888b-c365f6190ac5" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_2a4baa0c-d400-496c-872d-dc48305fc690" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_LesseeOperatingLeaseLiabilityToBePaidAfterYearFour_fe845770-42a9-46a8-a2cf-1b7d441af6a4" xlink:href="ctkb-20250331.xsd#ctkb_LesseeOperatingLeaseLiabilityToBePaidAfterYearFour"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_cecea64a-fedb-4a46-888b-c365f6190ac5" xlink:to="loc_ctkb_LesseeOperatingLeaseLiabilityToBePaidAfterYearFour_fe845770-42a9-46a8-a2cf-1b7d441af6a4" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails_1" xlink:type="simple" xlink:href="ctkb-20250331.xsd#LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails_1"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_e92d82c3-c449-40c2-bd5f-3d55d39e4c49" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_ca0c934a-587a-4c31-8cc7-777d1c461dc5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_e92d82c3-c449-40c2-bd5f-3d55d39e4c49" xlink:to="loc_us-gaap_OperatingLeaseLiability_ca0c934a-587a-4c31-8cc7-777d1c461dc5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_65101f1f-96d4-44d5-b209-65e3fbc9d7ff" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_e92d82c3-c449-40c2-bd5f-3d55d39e4c49" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_65101f1f-96d4-44d5-b209-65e3fbc9d7ff" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/CommitmentsandContingenciesDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#CommitmentsandContingenciesDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/CommitmentsandContingenciesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseObligation_f882ee9d-fd5e-4698-b752-5d602abecf78" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PurchaseObligation"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_PurchaseObligationToBePaidAfterYearTwo_47b1ae5a-0784-4b61-8725-03cb265940f6" xlink:href="ctkb-20250331.xsd#ctkb_PurchaseObligationToBePaidAfterYearTwo"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PurchaseObligation_f882ee9d-fd5e-4698-b752-5d602abecf78" xlink:to="loc_ctkb_PurchaseObligationToBePaidAfterYearTwo_47b1ae5a-0784-4b61-8725-03cb265940f6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseObligationDueInSecondYear_ae79eb21-3a44-4128-85b1-2c233e5ffe9c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PurchaseObligationDueInSecondYear"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PurchaseObligation_f882ee9d-fd5e-4698-b752-5d602abecf78" xlink:to="loc_us-gaap_PurchaseObligationDueInSecondYear_ae79eb21-3a44-4128-85b1-2c233e5ffe9c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseObligationDueInNextTwelveMonths_bef40d8e-71fc-4e2b-98fb-b5259fa6a795" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PurchaseObligationDueInNextTwelveMonths"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PurchaseObligation_f882ee9d-fd5e-4698-b752-5d602abecf78" xlink:to="loc_us-gaap_PurchaseObligationDueInNextTwelveMonths_bef40d8e-71fc-4e2b-98fb-b5259fa6a795" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PurchaseObligationFutureMinimumPaymentsRemainderOfFiscalYear_94500167-af44-4475-ba56-25d292222fe0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PurchaseObligationFutureMinimumPaymentsRemainderOfFiscalYear"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PurchaseObligation_f882ee9d-fd5e-4698-b752-5d602abecf78" xlink:to="loc_us-gaap_PurchaseObligationFutureMinimumPaymentsRemainderOfFiscalYear_94500167-af44-4475-ba56-25d292222fe0" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/NetLossAttributabletoCommonStockholdersPerShareScheduleofComputationoftheBasicandDilutedNetLossPerShareDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#NetLossAttributabletoCommonStockholdersPerShareScheduleofComputationoftheBasicandDilutedNetLossPerShareDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/NetLossAttributabletoCommonStockholdersPerShareScheduleofComputationoftheBasicandDilutedNetLossPerShareDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_39b48194-b6ad-4ab6-9d8b-e5492878305a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_74c45b7d-7e97-4410-8a69-31af10766b9e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_39b48194-b6ad-4ab6-9d8b-e5492878305a" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_74c45b7d-7e97-4410-8a69-31af10766b9e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncrementalCommonSharesAttributableToShareBasedPaymentArrangements_735c634b-be37-4650-8346-ece2812691c0" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncrementalCommonSharesAttributableToShareBasedPaymentArrangements"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_39b48194-b6ad-4ab6-9d8b-e5492878305a" xlink:to="loc_us-gaap_IncrementalCommonSharesAttributableToShareBasedPaymentArrangements_735c634b-be37-4650-8346-ece2812691c0" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://cytekbio.com/role/SegmentInformationandGeographicDataScheduleofSignificantSegmentExpensesDetails" xlink:type="simple" xlink:href="ctkb-20250331.xsd#SegmentInformationandGeographicDataScheduleofSignificantSegmentExpensesDetails"/>
  <link:calculationLink xlink:role="http://cytekbio.com/role/SegmentInformationandGeographicDataScheduleofSignificantSegmentExpensesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_97cc27af-5e56-4b42-8308-d3fd75ed72d3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_ResearchAndDevelopmentExpenseExcludingShareBasedPaymentArrangementExpense_f9228376-736d-4008-82c4-fbcfc4e6bb8c" xlink:href="ctkb-20250331.xsd#ctkb_ResearchAndDevelopmentExpenseExcludingShareBasedPaymentArrangementExpense"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_97cc27af-5e56-4b42-8308-d3fd75ed72d3" xlink:to="loc_ctkb_ResearchAndDevelopmentExpenseExcludingShareBasedPaymentArrangementExpense_f9228376-736d-4008-82c4-fbcfc4e6bb8c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_GeneralAndAdministrativeExpenseExcludingShareBasedPaymentArrangementExpense_e61427a0-db1a-4934-885e-27506a979c70" xlink:href="ctkb-20250331.xsd#ctkb_GeneralAndAdministrativeExpenseExcludingShareBasedPaymentArrangementExpense"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_97cc27af-5e56-4b42-8308-d3fd75ed72d3" xlink:to="loc_ctkb_GeneralAndAdministrativeExpenseExcludingShareBasedPaymentArrangementExpense_e61427a0-db1a-4934-885e-27506a979c70" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_8e972c14-7a6a-4534-b451-30feec44d9ab" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_97cc27af-5e56-4b42-8308-d3fd75ed72d3" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_8e972c14-7a6a-4534-b451-30feec44d9ab" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_MarketingExcludingShareBasedPaymentArrangementExpense_63c00836-6022-4790-aa30-dc0d4e7e4999" xlink:href="ctkb-20250331.xsd#ctkb_MarketingExcludingShareBasedPaymentArrangementExpense"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_97cc27af-5e56-4b42-8308-d3fd75ed72d3" xlink:to="loc_ctkb_MarketingExcludingShareBasedPaymentArrangementExpense_63c00836-6022-4790-aa30-dc0d4e7e4999" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingOtherItemAmount_222e338b-c9e2-4cec-8d69-c3a535c084f4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentReportingOtherItemAmount"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_97cc27af-5e56-4b42-8308-d3fd75ed72d3" xlink:to="loc_us-gaap_SegmentReportingOtherItemAmount_222e338b-c9e2-4cec-8d69-c3a535c084f4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_CostOfGoodsSold_3c536300-807c-42ce-8e15-a022f92ab491" xlink:href="ctkb-20250331.xsd#ctkb_CostOfGoodsSold"/>
    <link:calculationArc order="6" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_97cc27af-5e56-4b42-8308-d3fd75ed72d3" xlink:to="loc_ctkb_CostOfGoodsSold_3c536300-807c-42ce-8e15-a022f92ab491" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_00528dc0-97be-4cfb-af1a-6ba92e06c916" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_97cc27af-5e56-4b42-8308-d3fd75ed72d3" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_00528dc0-97be-4cfb-af1a-6ba92e06c916" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_SellingAndMarketingExpenseExcludingShareBasedPaymentArrangementExpense_b78f5d0b-a116-4c07-ad79-e6c285514f1d" xlink:href="ctkb-20250331.xsd#ctkb_SellingAndMarketingExpenseExcludingShareBasedPaymentArrangementExpense"/>
    <link:calculationArc order="8" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_97cc27af-5e56-4b42-8308-d3fd75ed72d3" xlink:to="loc_ctkb_SellingAndMarketingExpenseExcludingShareBasedPaymentArrangementExpense_b78f5d0b-a116-4c07-ad79-e6c285514f1d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_ctkb_AmortizationLicenseAndIPExpense_96f9706b-935b-499c-ad12-f1e336311183" xlink:href="ctkb-20250331.xsd#ctkb_AmortizationLicenseAndIPExpense"/>
    <link:calculationArc order="9" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_97cc27af-5e56-4b42-8308-d3fd75ed72d3" xlink:to="loc_ctkb_AmortizationLicenseAndIPExpense_96f9706b-935b-499c-ad12-f1e336311183" xlink:type="arc"/>
  </link:calculationLink>
</link:linkbase>
