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  <xs:element id="ctkb_IncreaseDecreaseInLegalSettlementLiabilities" abstract="false" name="IncreaseDecreaseInLegalSettlementLiabilities" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_TwoThousandAndTwentyOneEmployeeStockPurchasePlanMember" abstract="true" name="TwoThousandAndTwentyOneEmployeeStockPurchasePlanMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_AmnisTradenameMember" abstract="true" name="AmnisTradenameMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_AccountsReceivableAllowanceForCreditLossesPeriodIncreaseDecrease" abstract="false" name="AccountsReceivableAllowanceForCreditLossesPeriodIncreaseDecrease" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_SummaryOfUsefulLifeOfPropertyPlantAndEquipmentTableTextBlock" abstract="false" name="SummaryOfUsefulLifeOfPropertyPlantAndEquipmentTableTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:textBlockItemType"/>
  <xs:element id="ctkb_TwoThousandsTwentyTwoSalesAgreementMember" abstract="true" name="TwoThousandsTwentyTwoSalesAgreementMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_AccountingPoliciesTable" abstract="true" name="AccountingPoliciesTable" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrldt:hypercubeItem" type="xbrli:stringItemType"/>
  <xs:element id="ctkb_DeepcytoMember" abstract="true" name="DeepcytoMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_ScheduleOfPrepaidExpensesAndOtherCurrentAssetsTableTextBlock" abstract="false" name="ScheduleOfPrepaidExpensesAndOtherCurrentAssetsTableTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:textBlockItemType"/>
  <xs:element id="ctkb_NumberOfOptionForLeaseRenewal" abstract="false" name="NumberOfOptionForLeaseRenewal" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="xbrli:integerItemType"/>
  <xs:element id="ctkb_CommonStockTextBlock" abstract="false" name="CommonStockTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:textBlockItemType"/>
  <xs:element id="ctkb_RightOfUseAssetsObtainedInExchangeForNewLeaseObligations" abstract="false" name="RightOfUseAssetsObtainedInExchangeForNewLeaseObligations" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_UpfrontAndPaymentTerms" abstract="false" name="UpfrontAndPaymentTerms" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:durationItemType"/>
  <xs:element id="ctkb_Cashandcashequivalentsamortizedcostbasis" abstract="false" name="Cashandcashequivalentsamortizedcostbasis" nillable="true" xbrli:periodType="instant" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_AccumulatedMarketableSecuritiesUnrealizedGainLoss" abstract="false" name="AccumulatedMarketableSecuritiesUnrealizedGainLoss" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_CytekMember" abstract="true" name="CytekMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_FederalAgencySecuritiesMember" abstract="true" name="FederalAgencySecuritiesMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_ScheduleOfLegalSettlementLiabilityTableTextBlock" abstract="false" name="ScheduleOfLegalSettlementLiabilityTableTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:textBlockItemType"/>
  <xs:element id="ctkb_PrepaidTradeShow" abstract="false" name="PrepaidTradeShow" nillable="true" xbrli:periodType="instant" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_ShareBasedCompensationArrangementAnnualIncreaseInCommonStockReservedForIssuanceAsPercentageOfCommonStockOutstandingOnPrecedingFiscalYearEndDate" abstract="false" name="ShareBasedCompensationArrangementAnnualIncreaseInCommonStockReservedForIssuanceAsPercentageOfCommonStockOutstandingOnPrecedingFiscalYearEndDate" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="dtr-types1:percentItemType"/>
  <xs:element id="ctkb_StockOutstandingDuringPeriodSharesEmployeeStockPurchasePlans" abstract="false" name="StockOutstandingDuringPeriodSharesEmployeeStockPurchasePlans" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:sharesItemType"/>
  <xs:element id="ctkb_ScheduleOfIdentifiableIntangibleAssetsAcquiredAndTheirEstimatedUsefulLivesForAmortizationTableTextBlock" abstract="false" name="ScheduleOfIdentifiableIntangibleAssetsAcquiredAndTheirEstimatedUsefulLivesForAmortizationTableTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:textBlockItemType"/>
  <xs:element id="ctkb_SharesBuyBackValue" abstract="false" name="SharesBuyBackValue" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_ServiceContractsTerms" abstract="false" name="ServiceContractsTerms" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:durationItemType"/>
  <xs:element id="ctkb_BillAndHoldArrangementsMember" abstract="true" name="BillAndHoldArrangementsMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_LessThanOneYearMember" abstract="true" name="LessThanOneYearMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_ScheduleOfSupplementalBalanceSheetInformationRelatedToLeasesTableTextBlock" abstract="false" name="ScheduleOfSupplementalBalanceSheetInformationRelatedToLeasesTableTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:textBlockItemType"/>
  <xs:element id="ctkb_CytekJapanMember" abstract="true" name="CytekJapanMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_DiscountRate" abstract="false" name="DiscountRate" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="dtr-types1:percentItemType"/>
  <xs:element id="ctkb_LegalSettlementLiabilityCurrent" abstract="false" name="LegalSettlementLiabilityCurrent" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_OfficeFacilitiesAndEquipmentMember" abstract="true" name="OfficeFacilitiesAndEquipmentMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_PrepaidValueAddedTax" abstract="false" name="PrepaidValueAddedTax" nillable="true" xbrli:periodType="instant" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_AtMarketOfferingMember" abstract="true" name="AtMarketOfferingMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_RoyaltyPaymentDuration" abstract="false" name="RoyaltyPaymentDuration" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:durationItemType"/>
  <xs:element id="ctkb_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpiredInPeriod" abstract="false" name="ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpiredInPeriod" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:sharesItemType"/>
  <xs:element id="ctkb_ContractLiabilityPolicyPolicyTextBlock" abstract="false" name="ContractLiabilityPolicyPolicyTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:textBlockItemType"/>
  <xs:element id="ctkb_ReagentLicenseMember" abstract="true" name="ReagentLicenseMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_CostOfSalesRelatedToRoyaltyExpense" abstract="false" name="CostOfSalesRelatedToRoyaltyExpense" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_SettlementLiabilitiesMember" abstract="true" name="SettlementLiabilitiesMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_LuminexCorporationMember" abstract="true" name="LuminexCorporationMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_FciBusinessUnitMember" abstract="true" name="FciBusinessUnitMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_OperatingCashOutflowsPaymentsOnOperatingLeases" abstract="false" name="OperatingCashOutflowsPaymentsOnOperatingLeases" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_GreaterThanOneYearMember" abstract="true" name="GreaterThanOneYearMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_OtherCurrentLiabilitiesOther" abstract="false" name="OtherCurrentLiabilitiesOther" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_AccountingPoliciesLineItems" abstract="true" name="AccountingPoliciesLineItems" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:stringItemType"/>
  <xs:element id="ctkb_IntangibleAssetInAccruedExpense" abstract="false" name="IntangibleAssetInAccruedExpense" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_GuavaEasycyteMuseDevelopedTechnologyMember" abstract="true" name="GuavaEasycyteMuseDevelopedTechnologyMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_LitigationSettlementInterestPaymentPeriodDuration" abstract="false" name="LitigationSettlementInterestPaymentPeriodDuration" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="xbrli:durationItemType"/>
  <xs:element id="ctkb_AmnisImageStreamDevelopedTechnologyMember" abstract="true" name="AmnisImageStreamDevelopedTechnologyMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_PaymentsForTaxesRelatedToNetShareSettlementOfEquityAwards" abstract="false" name="PaymentsForTaxesRelatedToNetShareSettlementOfEquityAwards" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_OfficeAndComputerEquipmentMember" abstract="true" name="OfficeAndComputerEquipmentMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_LegalSettlementLiabilityNonCurrent" abstract="false" name="LegalSettlementLiabilityNonCurrent" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_LossGainOnInvestmentsAccretionAndAmortizationNet" abstract="false" name="LossGainOnInvestmentsAccretionAndAmortizationNet" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_BdMember" abstract="true" name="BdMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_EmployeeStockPurchasePlanTwoThousandAndTwentyOnePlanMember" abstract="true" name="EmployeeStockPurchasePlanTwoThousandAndTwentyOnePlanMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_AccruedPurchases" abstract="false" name="AccruedPurchases" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_LegalSettlementLiability" abstract="false" name="LegalSettlementLiability" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_NumberOfVotePerShare" abstract="false" name="NumberOfVotePerShare" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="xbrli:integerItemType"/>
  <xs:element id="ctkb_NoncontrollingInterestInConsolidatedSubsidiary" abstract="false" name="NoncontrollingInterestInConsolidatedSubsidiary" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_AcademiaAndGovernmentMember" abstract="true" name="AcademiaAndGovernmentMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_ReagentLicensesMember" abstract="true" name="ReagentLicensesMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_FciDevelopedTechnologyMember" abstract="true" name="FciDevelopedTechnologyMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_IPLicenseMember" abstract="true" name="IPLicenseMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_PortionOfLoanIncludedInCurrentLiabilities" abstract="false" name="PortionOfLoanIncludedInCurrentLiabilities" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_InterestExpensesForAccretionOfLegalSettlementLiabilities" abstract="false" name="InterestExpensesForAccretionOfLegalSettlementLiabilities" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_AmnisFlowSightCellStreamDevelopedTechnologyMember" abstract="true" name="AmnisFlowSightCellStreamDevelopedTechnologyMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_GeographicAreaDisclosureTextBlock" abstract="false" name="GeographicAreaDisclosureTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:textBlockItemType"/>
  <xs:element id="ctkb_PaymentForAdditionalInvestmentInCytekJapanNetOfCashAcquired" abstract="false" name="PaymentForAdditionalInvestmentInCytekJapanNetOfCashAcquired" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_CommonStockWithheldRelatedToNetShareSettlementShares" abstract="false" name="CommonStockWithheldRelatedToNetShareSettlementShares" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:sharesItemType"/>
  <xs:element id="ctkb_EmployeeStockPurchasePlanMember" abstract="true" name="EmployeeStockPurchasePlanMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_BusinessCombinationProvisionalInformationInitialAccountingIncompleteAdjustmentGoodwill" abstract="false" name="BusinessCombinationProvisionalInformationInitialAccountingIncompleteAdjustmentGoodwill" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_CustomerDepositLiabilityCurrent" abstract="false" name="CustomerDepositLiabilityCurrent" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_RightOfUseAssetsObtainedInExchangeForNewLeaseObligationsAbstract" abstract="true" name="RightOfUseAssetsObtainedInExchangeForNewLeaseObligationsAbstract" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:stringItemType"/>
  <xs:element id="ctkb_UpfrontPayment" abstract="false" name="UpfrontPayment" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_PatentsAndTrademarksMember" abstract="true" name="PatentsAndTrademarksMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_OtherMember" abstract="true" name="OtherMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_ScheduleOfSupplementalCashFlowInformationRelatedToLeasesTableTextBlock" abstract="false" name="ScheduleOfSupplementalCashFlowInformationRelatedToLeasesTableTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:textBlockItemType"/>
  <xs:element id="ctkb_CommonStockWithheldRelatedToNetshareSettlement" abstract="false" name="CommonStockWithheldRelatedToNetshareSettlement" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_TwoThousandAndTwentyOneEquityIncentivePlanMember" abstract="true" name="TwoThousandAndTwentyOneEquityIncentivePlanMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types1:domainItemType"/>
  <xs:element id="ctkb_LesseeOperatingLeaseLiabilityToBePaidAfterYearFour" abstract="false" name="LesseeOperatingLeaseLiabilityToBePaidAfterYearFour" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_ProceedsFromEmployeeStockPurchasePlans" abstract="false" name="ProceedsFromEmployeeStockPurchasePlans" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
</xs:schema>
