<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2025 Workiva-->
<!--r:a2ca81f3-6b3e-4bd5-8f05-2155743af73b,g:62604e95-eba2-4379-8ae4-04d859a2d57e-->
<xs:schema xmlns:xs="http://www.w3.org/2001/XMLSchema" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:ctkb="http://cytekbio.com/20250630" xmlns:xbrli="http://www.xbrl.org/2003/instance" xmlns:dtr-types="http://www.xbrl.org/dtr/type/2024-01-31" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" attributeFormDefault="unqualified" elementFormDefault="qualified" targetNamespace="http://cytekbio.com/20250630">
  <xs:import namespace="http://fasb.org/srt/2025" schemaLocation="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd"/>
  <xs:import namespace="http://fasb.org/us-gaap/2025" schemaLocation="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd"/>
  <xs:import namespace="http://www.xbrl.org/2003/instance" schemaLocation="http://www.xbrl.org/2003/xbrl-instance-2003-12-31.xsd"/>
  <xs:import namespace="http://www.xbrl.org/2003/linkbase" schemaLocation="http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd"/>
  <xs:import namespace="http://www.xbrl.org/dtr/type/2024-01-31" schemaLocation="https://www.xbrl.org/dtr/type/2024-01-31/types.xsd"/>
  <xs:import namespace="http://xbrl.org/2005/xbrldt" schemaLocation="http://www.xbrl.org/2005/xbrldt-2005.xsd"/>
  <xs:import namespace="http://xbrl.sec.gov/country/2025" schemaLocation="https://xbrl.sec.gov/country/2025/country-2025.xsd"/>
  <xs:import namespace="http://xbrl.sec.gov/dei/2025" schemaLocation="https://xbrl.sec.gov/dei/2025/dei-2025.xsd"/>
  <xs:import namespace="http://xbrl.sec.gov/ecd/2025" schemaLocation="https://xbrl.sec.gov/ecd/2025/ecd-2025.xsd"/>
  <xs:annotation>
    <xs:appinfo>
      <link:linkbaseRef xmlns:xlink="http://www.w3.org/1999/xlink" xlink:arcrole="http://www.w3.org/1999/xlink/properties/linkbase" xlink:href="ctkb-20250630_pre.xml" xlink:role="http://www.xbrl.org/2003/role/presentationLinkbaseRef" xlink:type="simple"/>
      <link:linkbaseRef xmlns:xlink="http://www.w3.org/1999/xlink" xlink:arcrole="http://www.w3.org/1999/xlink/properties/linkbase" xlink:href="ctkb-20250630_def.xml" xlink:role="http://www.xbrl.org/2003/role/definitionLinkbaseRef" xlink:type="simple"/>
      <link:linkbaseRef xmlns:xlink="http://www.w3.org/1999/xlink" xlink:arcrole="http://www.w3.org/1999/xlink/properties/linkbase" xlink:href="ctkb-20250630_lab.xml" xlink:role="http://www.xbrl.org/2003/role/labelLinkbaseRef" xlink:type="simple"/>
      <link:linkbaseRef xmlns:xlink="http://www.w3.org/1999/xlink" xlink:arcrole="http://www.w3.org/1999/xlink/properties/linkbase" xlink:href="ctkb-20250630_cal.xml" xlink:role="http://www.xbrl.org/2003/role/calculationLinkbaseRef" xlink:type="simple"/>
      <link:roleType id="Cover" roleURI="http://cytekbio.com/role/Cover">
        <link:definition>0000001 - Document - Cover</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="ConsolidatedBalanceSheets" roleURI="http://cytekbio.com/role/ConsolidatedBalanceSheets">
        <link:definition>9952151 - Statement - Consolidated Balance Sheets</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="ConsolidatedBalanceSheetsParenthetical" roleURI="http://cytekbio.com/role/ConsolidatedBalanceSheetsParenthetical">
        <link:definition>9952152 - Statement - Consolidated Balance Sheets (Parenthetical)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="ConsolidatedStatementsofOperationsandComprehensiveLossunaudited" roleURI="http://cytekbio.com/role/ConsolidatedStatementsofOperationsandComprehensiveLossunaudited">
        <link:definition>9952153 - Statement - Consolidated Statements of Operations and Comprehensive Loss (unaudited)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="ConsolidatedStatementsofStockholdersEquityUnaudited" roleURI="http://cytekbio.com/role/ConsolidatedStatementsofStockholdersEquityUnaudited">
        <link:definition>9952154 - Statement - Consolidated Statements of Stockholders&#8217; Equity (Unaudited)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="ConsolidatedStatementsofCashFlowsunaudited" roleURI="http://cytekbio.com/role/ConsolidatedStatementsofCashFlowsunaudited">
        <link:definition>9952155 - Statement - Consolidated Statements of Cash Flows (unaudited)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="DescriptionofBusiness" roleURI="http://cytekbio.com/role/DescriptionofBusiness">
        <link:definition>9952156 - Disclosure - Description of Business</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="BasisofPresentationandSummaryofSignificantAccountingPolicies" roleURI="http://cytekbio.com/role/BasisofPresentationandSummaryofSignificantAccountingPolicies">
        <link:definition>9952157 - Disclosure - Basis of Presentation and Summary of Significant Accounting Policies</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="ConcentrationsofCreditRiskandOtherRisksandUncertainties" roleURI="http://cytekbio.com/role/ConcentrationsofCreditRiskandOtherRisksandUncertainties">
        <link:definition>9952158 - Disclosure - Concentrations of Credit Risk and Other Risks and Uncertainties</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="RevenuefromContractswithCustomers" roleURI="http://cytekbio.com/role/RevenuefromContractswithCustomers">
        <link:definition>9952159 - Disclosure - Revenue from Contracts with Customers</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="BalanceSheetDetails" roleURI="http://cytekbio.com/role/BalanceSheetDetails">
        <link:definition>9952160 - Disclosure - Balance Sheet Details</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="FairValueofFinancialInstruments" roleURI="http://cytekbio.com/role/FairValueofFinancialInstruments">
        <link:definition>9952161 - Disclosure - Fair Value of Financial Instruments</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="Investments" roleURI="http://cytekbio.com/role/Investments">
        <link:definition>9952162 - Disclosure - Investments</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="PropertyandEquipmentNet" roleURI="http://cytekbio.com/role/PropertyandEquipmentNet">
        <link:definition>9952163 - Disclosure - Property and Equipment, Net</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="Acquisition" roleURI="http://cytekbio.com/role/Acquisition">
        <link:definition>9952164 - Disclosure - Acquisition</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="GoodwillandIntangibleAssetsNet" roleURI="http://cytekbio.com/role/GoodwillandIntangibleAssetsNet">
        <link:definition>9952165 - Disclosure - Goodwill and Intangible Assets, Net</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="LegalSettlementLiability" roleURI="http://cytekbio.com/role/LegalSettlementLiability">
        <link:definition>9952166 - Disclosure - Legal Settlement Liability</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="Debt" roleURI="http://cytekbio.com/role/Debt">
        <link:definition>9952167 - Disclosure - Debt</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="CommonStock" roleURI="http://cytekbio.com/role/CommonStock">
        <link:definition>9952168 - Disclosure - Common Stock</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="StockBasedCompensationPlan" roleURI="http://cytekbio.com/role/StockBasedCompensationPlan">
        <link:definition>9952169 - Disclosure - Stock-Based Compensation Plan</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="EmployeeBenefitPlan" roleURI="http://cytekbio.com/role/EmployeeBenefitPlan">
        <link:definition>9952170 - Disclosure - Employee Benefit Plan</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="IncomeTaxes" roleURI="http://cytekbio.com/role/IncomeTaxes">
        <link:definition>9952171 - Disclosure - Income Taxes</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="Lease" roleURI="http://cytekbio.com/role/Lease">
        <link:definition>9952172 - Disclosure - Lease</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="CommitmentsandContingencies" roleURI="http://cytekbio.com/role/CommitmentsandContingencies">
        <link:definition>9952173 - Disclosure - Commitments and Contingencies</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="ProductWarranty" roleURI="http://cytekbio.com/role/ProductWarranty">
        <link:definition>9952174 - Disclosure - Product Warranty</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="NetLossAttributabletoCommonStockholdersPerShare" roleURI="http://cytekbio.com/role/NetLossAttributabletoCommonStockholdersPerShare">
        <link:definition>9952175 - Disclosure - Net Loss Attributable to Common Stockholders Per Share</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="SegmentInformationandGeographicData" roleURI="http://cytekbio.com/role/SegmentInformationandGeographicData">
        <link:definition>9952176 - Disclosure - Segment Information and Geographic Data</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="SubsequentEvent" roleURI="http://cytekbio.com/role/SubsequentEvent">
        <link:definition>9952177 - Disclosure - Subsequent Event</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="BasisofPresentationandSummaryofSignificantAccountingPoliciesPolicies" roleURI="http://cytekbio.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesPolicies">
        <link:definition>9955511 - Disclosure - Basis of Presentation and Summary of Significant Accounting Policies (Policies)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="BasisofPresentationandSummaryofSignificantAccountingPoliciesTables" roleURI="http://cytekbio.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesTables">
        <link:definition>9955512 - Disclosure - Basis of Presentation and Summary of Significant Accounting Policies (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="RevenuefromContractswithCustomersTables" roleURI="http://cytekbio.com/role/RevenuefromContractswithCustomersTables">
        <link:definition>9955513 - Disclosure - Revenue from Contracts with Customers (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="BalanceSheetDetailsTables" roleURI="http://cytekbio.com/role/BalanceSheetDetailsTables">
        <link:definition>9955514 - Disclosure - Balance Sheet Details (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="FairValueofFinancialInstrumentsTables" roleURI="http://cytekbio.com/role/FairValueofFinancialInstrumentsTables">
        <link:definition>9955515 - Disclosure - Fair Value of Financial Instruments (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="InvestmentsTables" roleURI="http://cytekbio.com/role/InvestmentsTables">
        <link:definition>9955516 - Disclosure - Investments (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="PropertyandEquipmentNetTables" roleURI="http://cytekbio.com/role/PropertyandEquipmentNetTables">
        <link:definition>9955517 - Disclosure - Property and Equipment, Net (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="AcquisitionTables" roleURI="http://cytekbio.com/role/AcquisitionTables">
        <link:definition>9955518 - Disclosure - Acquisition (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="GoodwillandIntangibleAssetsNetTables" roleURI="http://cytekbio.com/role/GoodwillandIntangibleAssetsNetTables">
        <link:definition>9955519 - Disclosure - Goodwill and Intangible Assets, Net (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="LegalSettlementLiabilityTables" roleURI="http://cytekbio.com/role/LegalSettlementLiabilityTables">
        <link:definition>9955520 - Disclosure - Legal Settlement Liability (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="StockBasedCompensationPlanTables" roleURI="http://cytekbio.com/role/StockBasedCompensationPlanTables">
        <link:definition>9955521 - Disclosure - Stock-Based Compensation Plan (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="IncomeTaxesTables" roleURI="http://cytekbio.com/role/IncomeTaxesTables">
        <link:definition>9955522 - Disclosure - Income Taxes (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="LeaseTables" roleURI="http://cytekbio.com/role/LeaseTables">
        <link:definition>9955523 - Disclosure - Lease (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="CommitmentandContingenciesTables" roleURI="http://cytekbio.com/role/CommitmentandContingenciesTables">
        <link:definition>9955524 - Disclosure - Commitment and Contingencies (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="ProductWarrantyTables" roleURI="http://cytekbio.com/role/ProductWarrantyTables">
        <link:definition>9955525 - Disclosure - Product Warranty (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="NetLossAttributabletoCommonStockholdersPerShareTables" roleURI="http://cytekbio.com/role/NetLossAttributabletoCommonStockholdersPerShareTables">
        <link:definition>9955526 - Disclosure - Net Loss Attributable to Common Stockholders Per Share (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="SegmentInformationandGeographicDataTables" roleURI="http://cytekbio.com/role/SegmentInformationandGeographicDataTables">
        <link:definition>9955527 - Disclosure - Segment Information and Geographic Data (Tables)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="BasisofPresentationandSummaryofSignificantAccountingPoliciesAdditionalInformationDetails" roleURI="http://cytekbio.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesAdditionalInformationDetails">
        <link:definition>9955528 - Disclosure - Basis of Presentation and Summary of Significant Accounting Policies - Additional Information (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="BasisofPresentationandSummaryofSignificantAccountingPoliciesScheduleofCashCashEquivalentsandRestrictedCashDetails" roleURI="http://cytekbio.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesScheduleofCashCashEquivalentsandRestrictedCashDetails">
        <link:definition>9955529 - Disclosure - Basis of Presentation and Summary of Significant Accounting Policies - Schedule of Cash, Cash Equivalents and Restricted Cash (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="BasisofPresentationandSummaryofSignificantAccountingPoliciesScheduleofEstimatedUsefulLifeofPropertyandEquipmentDetails" roleURI="http://cytekbio.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesScheduleofEstimatedUsefulLifeofPropertyandEquipmentDetails">
        <link:definition>9955530 - Disclosure - Basis of Presentation and Summary of Significant Accounting Policies - Schedule of Estimated Useful Life of Property and Equipment (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="BasisofPresentationandSummaryofSignificantAccountingPoliciesScheduleofEstimatedUsefulLivesofitsIntangibleAssetsDetails" roleURI="http://cytekbio.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesScheduleofEstimatedUsefulLivesofitsIntangibleAssetsDetails">
        <link:definition>9955531 - Disclosure - Basis of Presentation and Summary of Significant Accounting Policies - Schedule of Estimated Useful Lives of its Intangible Assets (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="RevenuefromContractswithCustomersScheduleofDisaggregationofRevenueDetails" roleURI="http://cytekbio.com/role/RevenuefromContractswithCustomersScheduleofDisaggregationofRevenueDetails">
        <link:definition>9955532 - Disclosure - Revenue from Contracts with Customers - Schedule of Disaggregation of Revenue (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="RevenuefromContractswithCustomersScheduleofEstimatedRevenuesExpectedtobeRecognizedintheFutureRelatedtoPerformanceObligationsDetails" roleURI="http://cytekbio.com/role/RevenuefromContractswithCustomersScheduleofEstimatedRevenuesExpectedtobeRecognizedintheFutureRelatedtoPerformanceObligationsDetails">
        <link:definition>9955533 - Disclosure - Revenue from Contracts with Customers - Schedule of Estimated Revenues Expected to be Recognized in the Future Related to Performance Obligations (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="RevenuefromContractswithCustomersScheduleofInformationAboutReceivablesDeferredRevenueFromContractsWithCustomersandCustomerDepositsDetails" roleURI="http://cytekbio.com/role/RevenuefromContractswithCustomersScheduleofInformationAboutReceivablesDeferredRevenueFromContractsWithCustomersandCustomerDepositsDetails">
        <link:definition>9955534 - Disclosure - Revenue from Contracts with Customers - Schedule of Information About Receivables, Deferred Revenue From Contracts With Customers and Customer Deposits (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="RevenuefromContractswithCustomersScheduleofRollforwardofCurrentLiabilitiesDetails" roleURI="http://cytekbio.com/role/RevenuefromContractswithCustomersScheduleofRollforwardofCurrentLiabilitiesDetails">
        <link:definition>9955535 - Disclosure - Revenue from Contracts with Customers - Schedule of Rollforward of Current Liabilities (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="BalanceSheetDetailsScheduleofComponentsofInventoryDetails" roleURI="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofInventoryDetails">
        <link:definition>9955536 - Disclosure - Balance Sheet Details - Schedule of Components of Inventory (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="BalanceSheetDetailsScheduleofComponentsofPrepaidExpensesandOtherCurrentAssetsDetails" roleURI="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofPrepaidExpensesandOtherCurrentAssetsDetails">
        <link:definition>9955537 - Disclosure - Balance Sheet Details - Schedule of Components of Prepaid Expenses and Other Current Assets (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="BalanceSheetDetailsScheduleofComponentsofAccruedExpensesDetails" roleURI="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofAccruedExpensesDetails">
        <link:definition>9955538 - Disclosure - Balance Sheet Details - Schedule of Components of Accrued Expenses (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="BalanceSheetDetailsScheduleofComponentsofOtherCurrentLiabilitiesDetails" roleURI="http://cytekbio.com/role/BalanceSheetDetailsScheduleofComponentsofOtherCurrentLiabilitiesDetails">
        <link:definition>9955539 - Disclosure - Balance Sheet Details - Schedule of Components of Other Current Liabilities (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="FairValueofFinancialInstrumentsScheduleofFinancialInstrumentsMeasuredonRecurringBasisDetails" roleURI="http://cytekbio.com/role/FairValueofFinancialInstrumentsScheduleofFinancialInstrumentsMeasuredonRecurringBasisDetails">
        <link:definition>9955540 - Disclosure - Fair Value of Financial Instruments - Schedule of Financial Instruments Measured on Recurring Basis (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="FairValueofFinancialInstrumentsAdditionalInformationDetails" roleURI="http://cytekbio.com/role/FairValueofFinancialInstrumentsAdditionalInformationDetails">
        <link:definition>9955541 - Disclosure - Fair Value of Financial Instruments - Additional Information (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="InvestmentsScheduleofInvestmentsinAvailableforSaleSecuritiesDetails" roleURI="http://cytekbio.com/role/InvestmentsScheduleofInvestmentsinAvailableforSaleSecuritiesDetails">
        <link:definition>9955542 - Disclosure - Investments - Schedule of Investments in Available-for-Sale Securities (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="InvestmentsScheduleoftheContractualMaturitiesofAvailableforSaleSecuritiesDetails" roleURI="http://cytekbio.com/role/InvestmentsScheduleoftheContractualMaturitiesofAvailableforSaleSecuritiesDetails">
        <link:definition>9955543 - Disclosure - Investments - Schedule of the Contractual Maturities of Available-for-Sale Securities (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="PropertyandEquipmentNetScheduleofComponentsofPropertyandEquipmentNetDetails" roleURI="http://cytekbio.com/role/PropertyandEquipmentNetScheduleofComponentsofPropertyandEquipmentNetDetails">
        <link:definition>9955544 - Disclosure - Property and Equipment, Net - Schedule of Components of Property and Equipment, Net (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="PropertyandEquipmentNetAdditionalInformationDetails" roleURI="http://cytekbio.com/role/PropertyandEquipmentNetAdditionalInformationDetails">
        <link:definition>9955545 - Disclosure - Property and Equipment, Net - Additional Information (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="AcquisitionAdditionalInformationDetails" roleURI="http://cytekbio.com/role/AcquisitionAdditionalInformationDetails">
        <link:definition>9955546 - Disclosure - Acquisition - Additional Information (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="AcquisitionScheduleofEstimatedFairValueofAssetsAcquiredandLiabilitiesAssumedDetails" roleURI="http://cytekbio.com/role/AcquisitionScheduleofEstimatedFairValueofAssetsAcquiredandLiabilitiesAssumedDetails">
        <link:definition>9955547 - Disclosure - Acquisition - Schedule of Estimated Fair Value of Assets Acquired and Liabilities Assumed (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="AcquisitionScheduleofIdentifiableIntangibleAssetsAcquiredandTheirEstimatedUsefulLivesforAmortizationDetails" roleURI="http://cytekbio.com/role/AcquisitionScheduleofIdentifiableIntangibleAssetsAcquiredandTheirEstimatedUsefulLivesforAmortizationDetails">
        <link:definition>9955548 - Disclosure - Acquisition - Schedule of Identifiable Intangible Assets Acquired and Their Estimated Useful Lives for Amortization (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="GoodwillandIntangibleAssetsNetScheduleofComponentsofIntangibleAssetsNetDetails" roleURI="http://cytekbio.com/role/GoodwillandIntangibleAssetsNetScheduleofComponentsofIntangibleAssetsNetDetails">
        <link:definition>9955549 - Disclosure - Goodwill and Intangible Assets, Net - Schedule of Components of Intangible Assets, Net (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="GoodwillandIntangibleAssetsNetAdditionalInformationDetails" roleURI="http://cytekbio.com/role/GoodwillandIntangibleAssetsNetAdditionalInformationDetails">
        <link:definition>9955550 - Disclosure - Goodwill and Intangible Assets, Net - Additional Information (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="LegalSettlementLiabilityAdditionalInformationDetails" roleURI="http://cytekbio.com/role/LegalSettlementLiabilityAdditionalInformationDetails">
        <link:definition>9955551 - Disclosure - Legal Settlement Liability - Additional Information (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="LegalSettlementLiabilityScheduleofLegalSettlementLiabilityDetails" roleURI="http://cytekbio.com/role/LegalSettlementLiabilityScheduleofLegalSettlementLiabilityDetails">
        <link:definition>9955552 - Disclosure - Legal Settlement Liability - Schedule of Legal Settlement Liability (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="DebtDetails" roleURI="http://cytekbio.com/role/DebtDetails">
        <link:definition>9955553 - Disclosure - Debt (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="CommonStockDetails" roleURI="http://cytekbio.com/role/CommonStockDetails">
        <link:definition>9955554 - Disclosure - Common Stock (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="StockBasedCompensationPlanAdditionalInformationDetails" roleURI="http://cytekbio.com/role/StockBasedCompensationPlanAdditionalInformationDetails">
        <link:definition>9955555 - Disclosure - Stock-Based Compensation Plan - Additional Information (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="StockBasedCompensationPlanScheduleofStockOptionActivityDetails" roleURI="http://cytekbio.com/role/StockBasedCompensationPlanScheduleofStockOptionActivityDetails">
        <link:definition>9955556 - Disclosure - Stock-Based Compensation Plan - Schedule of Stock Option Activity (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="StockBasedCompensationPlanScheduleofRSUAwardsActivityDetails" roleURI="http://cytekbio.com/role/StockBasedCompensationPlanScheduleofRSUAwardsActivityDetails">
        <link:definition>9955557 - Disclosure - Stock-Based Compensation Plan - Schedule of RSU Awards Activity (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="StockBasedCompensationPlanScheduleofAllocationofStockBasedCompensationExpenseDetails" roleURI="http://cytekbio.com/role/StockBasedCompensationPlanScheduleofAllocationofStockBasedCompensationExpenseDetails">
        <link:definition>9955558 - Disclosure - Stock-Based Compensation Plan - Schedule of Allocation of Stock-Based Compensation Expense (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="StockBasedCompensationPlanScheduleofWeightedAverageValuationAssumptionsUsedinDeterminingtheFairValueofEmployeeStockOptionsDetails" roleURI="http://cytekbio.com/role/StockBasedCompensationPlanScheduleofWeightedAverageValuationAssumptionsUsedinDeterminingtheFairValueofEmployeeStockOptionsDetails">
        <link:definition>9955559 - Disclosure - Stock-Based Compensation Plan - Schedule of Weighted Average Valuation Assumptions Used in Determining the Fair Value of Employee Stock Options (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="StockBasedCompensationPlanScheduleofWeightedAverageValuationAssumptionsUsedinEstimatingtheFairValueofESPPDetails" roleURI="http://cytekbio.com/role/StockBasedCompensationPlanScheduleofWeightedAverageValuationAssumptionsUsedinEstimatingtheFairValueofESPPDetails">
        <link:definition>9955560 - Disclosure - Stock-Based Compensation Plan - Schedule of Weighted Average Valuation Assumptions Used in Estimating the Fair Value of ESPP (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="EmployeeBenefitPlanDetails" roleURI="http://cytekbio.com/role/EmployeeBenefitPlanDetails">
        <link:definition>9955561 - Disclosure - Employee Benefit Plan (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="IncomeTaxesAdditionalInformationDetails" roleURI="http://cytekbio.com/role/IncomeTaxesAdditionalInformationDetails">
        <link:definition>9955562 - Disclosure - Income Taxes - Additional Information (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="IncomeTaxesScheduleofBenefitfromIncomeTaxesDetails" roleURI="http://cytekbio.com/role/IncomeTaxesScheduleofBenefitfromIncomeTaxesDetails">
        <link:definition>9955563 - Disclosure - Income Taxes - Schedule of Benefit from Income Taxes (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="LeaseAdditionalInformationDetails" roleURI="http://cytekbio.com/role/LeaseAdditionalInformationDetails">
        <link:definition>9955564 - Disclosure - Lease - Additional Information (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="LeaseScheduleofComponentsofLeaseExpenseDetails" roleURI="http://cytekbio.com/role/LeaseScheduleofComponentsofLeaseExpenseDetails">
        <link:definition>9955565 - Disclosure - Lease - Schedule of Components of Lease Expense (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="LeaseScheduleofSupplementalCashFlowInformationRelatedtoLeasesDetails" roleURI="http://cytekbio.com/role/LeaseScheduleofSupplementalCashFlowInformationRelatedtoLeasesDetails">
        <link:definition>9955566 - Disclosure - Lease - Schedule of Supplemental Cash Flow Information Related to Leases (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="LeaseScheduleofSupplementalBalanceSheetInformationRelatedtoLeasesDetails" roleURI="http://cytekbio.com/role/LeaseScheduleofSupplementalBalanceSheetInformationRelatedtoLeasesDetails">
        <link:definition>9955567 - Disclosure - Lease - Schedule of Supplemental Balance Sheet Information Related to Leases (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails" roleURI="http://cytekbio.com/role/LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails">
        <link:definition>9955568 - Disclosure - Lease - Schedule of Future Undiscounted Cash Flows and Reconciliation to the Lease Liabilities Recognized (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails_1" roleURI="http://cytekbio.com/role/LeaseScheduleofFutureUndiscountedCashFlowsandReconciliationtotheLeaseLiabilitiesRecognizedDetails_1">
        <link:definition>9955568 - Disclosure - Lease - Schedule of Future Undiscounted Cash Flows and Reconciliation to the Lease Liabilities Recognized (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="CommitmentsandContingenciesDetails" roleURI="http://cytekbio.com/role/CommitmentsandContingenciesDetails">
        <link:definition>9955569 - Disclosure - Commitments and Contingencies (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="ProductWarrantyDetails" roleURI="http://cytekbio.com/role/ProductWarrantyDetails">
        <link:definition>9955570 - Disclosure - Product Warranty (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="NetLossAttributabletoCommonStockholdersPerShareScheduleofComputationoftheBasicandDilutedNetLossPerShareDetails" roleURI="http://cytekbio.com/role/NetLossAttributabletoCommonStockholdersPerShareScheduleofComputationoftheBasicandDilutedNetLossPerShareDetails">
        <link:definition>9955571 - Disclosure - Net Loss Attributable to Common Stockholders Per Share - Schedule of Computation of the Basic and Diluted Net Loss Per Share (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="NetLossAttributabletoCommonStockholdersPerShareAdditionalInformationDetails" roleURI="http://cytekbio.com/role/NetLossAttributabletoCommonStockholdersPerShareAdditionalInformationDetails">
        <link:definition>9955572 - Disclosure - Net Loss Attributable to Common Stockholders Per Share - Additional Information (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="SegmentInformationandGeographicDataAdditionalInformationDetails" roleURI="http://cytekbio.com/role/SegmentInformationandGeographicDataAdditionalInformationDetails">
        <link:definition>9955573 - Disclosure - Segment Information and Geographic Data - Additional Information (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="SegmentInformationandGeographicDataScheduleofSignificantSegmentExpensesDetails" roleURI="http://cytekbio.com/role/SegmentInformationandGeographicDataScheduleofSignificantSegmentExpensesDetails">
        <link:definition>9955574 - Disclosure - Segment Information and Geographic Data - Schedule of Significant Segment Expenses (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="SegmentInformationandGeographicDataScheduleofGeographicalDistributionofRevenueDetails" roleURI="http://cytekbio.com/role/SegmentInformationandGeographicDataScheduleofGeographicalDistributionofRevenueDetails">
        <link:definition>9955575 - Disclosure - Segment Information and Geographic Data - Schedule of Geographical Distribution of Revenue (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
      <link:roleType id="SegmentInformationandGeographicDataScheduleofLongLivedAssetsbyGeographicAreaDetails" roleURI="http://cytekbio.com/role/SegmentInformationandGeographicDataScheduleofLongLivedAssetsbyGeographicAreaDetails">
        <link:definition>9955576 - Disclosure - Segment Information and Geographic Data - Schedule of Long-Lived Assets by Geographic Area (Details)</link:definition>
        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
      </link:roleType>
    </xs:appinfo>
  </xs:annotation>
  <xs:element id="ctkb_CommonStockWithheldRelatedToNetShareSettlementShares" abstract="false" name="CommonStockWithheldRelatedToNetShareSettlementShares" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:sharesItemType"/>
  <xs:element id="ctkb_CommonStockWithheldRelatedToNetShareSettlementValue" abstract="false" name="CommonStockWithheldRelatedToNetShareSettlementValue" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_AccumulatedMarketableSecuritiesUnrealizedGainLoss" abstract="false" name="AccumulatedMarketableSecuritiesUnrealizedGainLoss" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_LossGainOnInvestmentsAccretionAndAmortizationNet" abstract="false" name="LossGainOnInvestmentsAccretionAndAmortizationNet" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_InterestExpensesForAccretionOfLegalSettlementLiabilities" abstract="false" name="InterestExpensesForAccretionOfLegalSettlementLiabilities" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_IncreaseDecreaseInLegalSettlementLiabilities" abstract="false" name="IncreaseDecreaseInLegalSettlementLiabilities" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_PurchasesOfMarketableSecurities" abstract="false" name="PurchasesOfMarketableSecurities" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_PaymentsForTaxesRelatedToNetShareSettlementOfEquityAwards" abstract="false" name="PaymentsForTaxesRelatedToNetShareSettlementOfEquityAwards" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_InternalInventoryTransferToFixedAssets" abstract="false" name="InternalInventoryTransferToFixedAssets" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_IntangibleAssetInAccruedExpense" abstract="false" name="IntangibleAssetInAccruedExpense" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_CommonStockTextBlock" abstract="false" name="CommonStockTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:textBlockItemType"/>
  <xs:element id="ctkb_ContractLiabilityPolicyPolicyTextBlock" abstract="false" name="ContractLiabilityPolicyPolicyTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:textBlockItemType"/>
  <xs:element id="ctkb_DeferredOfferingCostsPolicyPolicyTextBlock" abstract="false" name="DeferredOfferingCostsPolicyPolicyTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:textBlockItemType"/>
  <xs:element id="ctkb_SummaryOfUsefulLifeOfPropertyPlantAndEquipmentTableTextBlock" abstract="false" name="SummaryOfUsefulLifeOfPropertyPlantAndEquipmentTableTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:textBlockItemType"/>
  <xs:element id="ctkb_ScheduleOfPrepaidExpensesAndOtherCurrentAssetsTableTextBlock" abstract="false" name="ScheduleOfPrepaidExpensesAndOtherCurrentAssetsTableTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:textBlockItemType"/>
  <xs:element id="ctkb_ScheduleOfIdentifiableIntangibleAssetsAcquiredAndTheirEstimatedUsefulLivesForAmortizationTableTextBlock" abstract="false" name="ScheduleOfIdentifiableIntangibleAssetsAcquiredAndTheirEstimatedUsefulLivesForAmortizationTableTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:textBlockItemType"/>
  <xs:element id="ctkb_ScheduleOfLegalSettlementLiabilityTableTextBlock" abstract="false" name="ScheduleOfLegalSettlementLiabilityTableTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:textBlockItemType"/>
  <xs:element id="ctkb_ScheduleOfSupplementalCashFlowInformationRelatedToLeasesTableTextBlock" abstract="false" name="ScheduleOfSupplementalCashFlowInformationRelatedToLeasesTableTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:textBlockItemType"/>
  <xs:element id="ctkb_ScheduleOfSupplementalBalanceSheetInformationRelatedToLeasesTableTextBlock" abstract="false" name="ScheduleOfSupplementalBalanceSheetInformationRelatedToLeasesTableTextBlock" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:textBlockItemType"/>
  <xs:element id="ctkb_AccountingPoliciesTable" abstract="true" name="AccountingPoliciesTable" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrldt:hypercubeItem" type="xbrli:stringItemType"/>
  <xs:element id="ctkb_TwoThousandsTwentyTwoSalesAgreementMember" abstract="true" name="TwoThousandsTwentyTwoSalesAgreementMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_AtMarketOfferingMember" abstract="true" name="AtMarketOfferingMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_AccountingPoliciesLineItems" abstract="true" name="AccountingPoliciesLineItems" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:stringItemType"/>
  <xs:element id="ctkb_UpfrontAndPaymentTerms" abstract="false" name="UpfrontAndPaymentTerms" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:durationItemType"/>
  <xs:element id="ctkb_ServiceContractsTerms" abstract="false" name="ServiceContractsTerms" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:durationItemType"/>
  <xs:element id="ctkb_AggregateCompensationPayablePercentage" abstract="false" name="AggregateCompensationPayablePercentage" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="xbrli:pureItemType"/>
  <xs:element id="ctkb_FciDevelopedTechnologyMember" abstract="true" name="FciDevelopedTechnologyMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_ReagentLicensesMember" abstract="true" name="ReagentLicensesMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_AcademiaAndGovernmentMember" abstract="true" name="AcademiaAndGovernmentMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_BiotechnologyPharmaceuticalDistributorAndCROMember" abstract="true" name="BiotechnologyPharmaceuticalDistributorAndCROMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_LessThanOneYearMember" abstract="true" name="LessThanOneYearMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_GreaterThanOneYearMember" abstract="true" name="GreaterThanOneYearMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_CustomerDepositLiabilityCurrent" abstract="false" name="CustomerDepositLiabilityCurrent" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_ContractLiabilitiesRollForward" abstract="true" name="ContractLiabilitiesRollForward" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:stringItemType"/>
  <xs:element id="ctkb_ContractWithCustomerLiabilityRevenueRecognizedExcludingOpeningBalance" abstract="false" name="ContractWithCustomerLiabilityRevenueRecognizedExcludingOpeningBalance" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_PrepaidInventory" abstract="false" name="PrepaidInventory" nillable="true" xbrli:periodType="instant" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_TaxRefundReceivableAndPayable" abstract="false" name="TaxRefundReceivableAndPayable" nillable="true" xbrli:periodType="instant" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_AccruedPurchases" abstract="false" name="AccruedPurchases" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_OtherCurrentLiabilitiesOther" abstract="false" name="OtherCurrentLiabilitiesOther" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_MatureInLessThanOneYearMember" abstract="true" name="MatureInLessThanOneYearMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_LaboratoryEquipmentMember" abstract="true" name="LaboratoryEquipmentMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_OfficeAndComputerEquipmentMember" abstract="true" name="OfficeAndComputerEquipmentMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_FCIMember" abstract="true" name="FCIMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_AggregateCashConsideration" abstract="false" name="AggregateCashConsideration" nillable="true" xbrli:periodType="instant" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_BusinessCombinationProvisionalInformationInitialAccountingIncompleteAdjustmentDeferredTaxAssets" abstract="false" name="BusinessCombinationProvisionalInformationInitialAccountingIncompleteAdjustmentDeferredTaxAssets" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_BusinessCombinationProvisionalInformationInitialAccountingIncompleteAdjustmentGoodwill" abstract="false" name="BusinessCombinationProvisionalInformationInitialAccountingIncompleteAdjustmentGoodwill" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_DiscountRate" abstract="false" name="DiscountRate" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="dtr-types:percentItemType"/>
  <xs:element id="ctkb_AmnisImageStreamDevelopedTechnologyMember" abstract="true" name="AmnisImageStreamDevelopedTechnologyMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_GuavaEasycyteMuseDevelopedTechnologyMember" abstract="true" name="GuavaEasycyteMuseDevelopedTechnologyMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_AmnisFlowSightCellStreamDevelopedTechnologyMember" abstract="true" name="AmnisFlowSightCellStreamDevelopedTechnologyMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_AmnisTradenameMember" abstract="true" name="AmnisTradenameMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_GuavaTradenameMember" abstract="true" name="GuavaTradenameMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_PatentsAndTrademarksMember" abstract="true" name="PatentsAndTrademarksMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_IPLicenseMember" abstract="true" name="IPLicenseMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_ReagentLicenseMember" abstract="true" name="ReagentLicenseMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_BectonDickinsonAndCompanyVS.CytekMember" abstract="true" name="BectonDickinsonAndCompanyVS.CytekMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_UpfrontPayment" abstract="false" name="UpfrontPayment" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_RoyaltyPaymentDuration" abstract="false" name="RoyaltyPaymentDuration" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:durationItemType"/>
  <xs:element id="ctkb_MileStonePayment" abstract="false" name="MileStonePayment" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_FiniteLivedIntangibleAssetAverageUsefulLife" abstract="false" name="FiniteLivedIntangibleAssetAverageUsefulLife" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="xbrli:durationItemType"/>
  <xs:element id="ctkb_SettlementLiabilitiesPaymentPeriod" abstract="false" name="SettlementLiabilitiesPaymentPeriod" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="xbrli:durationItemType"/>
  <xs:element id="ctkb_LegalSettlementLiabilityCurrent" abstract="false" name="LegalSettlementLiabilityCurrent" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_LegalSettlementLiabilityNonCurrent" abstract="false" name="LegalSettlementLiabilityNonCurrent" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_LegalSettlementLiability" abstract="false" name="LegalSettlementLiability" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_CytekMember" abstract="true" name="CytekMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_PortionOfLoanIncludedInCurrentLiabilities" abstract="false" name="PortionOfLoanIncludedInCurrentLiabilities" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_ThreeRepurchaseProgramsMember" abstract="true" name="ThreeRepurchaseProgramsMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_NumberOfVotePerShare" abstract="false" name="NumberOfVotePerShare" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="xbrli:integerItemType"/>
  <xs:element id="ctkb_StockRepurchaseProgramCommissionCosts" abstract="false" name="StockRepurchaseProgramCommissionCosts" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_NumberOfShareRepurchaseProgram" abstract="false" name="NumberOfShareRepurchaseProgram" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="xbrli:integerItemType"/>
  <xs:element id="ctkb_TwoThousandAndTwentyOneEquityIncentivePlanMember" abstract="true" name="TwoThousandAndTwentyOneEquityIncentivePlanMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_A2021EmployeeStockPurchasePlanMember" abstract="true" name="A2021EmployeeStockPurchasePlanMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_NumberOfStockBasedCompensationPlan" abstract="false" name="NumberOfStockBasedCompensationPlan" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="xbrli:integerItemType"/>
  <xs:element id="ctkb_ShareBasedCompensationArrangementAnnualIncreaseInCommonStockReservedForIssuanceAsPercentageOfCommonStockOutstandingOnPrecedingFiscalYearEndDate" abstract="false" name="ShareBasedCompensationArrangementAnnualIncreaseInCommonStockReservedForIssuanceAsPercentageOfCommonStockOutstandingOnPrecedingFiscalYearEndDate" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="dtr-types:percentItemType"/>
  <xs:element id="ctkb_StockOutstandingDuringPeriodSharesEmployeeStockPurchasePlans" abstract="false" name="StockOutstandingDuringPeriodSharesEmployeeStockPurchasePlans" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:sharesItemType"/>
  <xs:element id="ctkb_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpiredInPeriod" abstract="false" name="ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpiredInPeriod" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:sharesItemType"/>
  <xs:element id="ctkb_EmployeeStockPurchasePlanMember" abstract="true" name="EmployeeStockPurchasePlanMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_IncomeTaxExpenseBenefitIncreaseDecrease" abstract="false" name="IncomeTaxExpenseBenefitIncreaseDecrease" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_IncomeTaxExpenseBenefitIncreaseDecreasePercent" abstract="false" name="IncomeTaxExpenseBenefitIncreaseDecreasePercent" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:percentItemType"/>
  <xs:element id="ctkb_OfficeFacilitiesAndEquipmentMember" abstract="true" name="OfficeFacilitiesAndEquipmentMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_NumberOfOptionForLeaseRenewal" abstract="false" name="NumberOfOptionForLeaseRenewal" nillable="true" xbrli:periodType="instant" substitutionGroup="xbrli:item" type="xbrli:integerItemType"/>
  <xs:element id="ctkb_OperatingCashOutflowsPaymentsOnOperatingLeases" abstract="false" name="OperatingCashOutflowsPaymentsOnOperatingLeases" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_RightOfUseAssetsObtainedInExchangeForNewLeaseObligationsAbstract" abstract="true" name="RightOfUseAssetsObtainedInExchangeForNewLeaseObligationsAbstract" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="xbrli:stringItemType"/>
  <xs:element id="ctkb_RightOfUseAssetsObtainedInExchangeForNewLeaseObligations" abstract="false" name="RightOfUseAssetsObtainedInExchangeForNewLeaseObligations" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_LesseeOperatingLeaseLiabilityToBePaidAfterYearFour" abstract="false" name="LesseeOperatingLeaseLiabilityToBePaidAfterYearFour" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_PurchaseObligationToBePaidAfterYearTwo" abstract="false" name="PurchaseObligationToBePaidAfterYearTwo" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_DilutiveShareMember" abstract="true" name="DilutiveShareMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_ReportableSegmentMember" abstract="true" name="ReportableSegmentMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
  <xs:element id="ctkb_CostOfGoodsSold" abstract="false" name="CostOfGoodsSold" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_AmortizationLicenseAndIPExpense" abstract="false" name="AmortizationLicenseAndIPExpense" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_SellingAndMarketingExpenseExcludingShareBasedPaymentArrangementExpense" abstract="false" name="SellingAndMarketingExpenseExcludingShareBasedPaymentArrangementExpense" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_MarketingExcludingShareBasedPaymentArrangementExpense" abstract="false" name="MarketingExcludingShareBasedPaymentArrangementExpense" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_ResearchAndDevelopmentExpenseExcludingShareBasedPaymentArrangementExpense" abstract="false" name="ResearchAndDevelopmentExpenseExcludingShareBasedPaymentArrangementExpense" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_GeneralAndAdministrativeExpenseExcludingShareBasedPaymentArrangementExpense" abstract="false" name="GeneralAndAdministrativeExpenseExcludingShareBasedPaymentArrangementExpense" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType"/>
  <xs:element id="ctkb_OtherMember" abstract="true" name="OtherMember" nillable="true" xbrli:periodType="duration" substitutionGroup="xbrli:item" type="dtr-types:domainItemType"/>
</xs:schema>
